Nozomi Yumehara, a regular middle school student, finds a magical book called the Dream Collet in the library and meets Coco, a creature from the Palmier Kingdom. Nozomi decides to help restore Coco's world, which has been destroyed by an organization called Nightmare, by completing the Dream Collet and finding the 55 Pinkies to make any wish come true. By making her decision to help Coco, she proves she has the heart to be a Pretty Cure and is given her Pinky Catch from a pink butterfly letting her transform into Cure Dream. Meanwhile, the Nightmares are fighting the cures to steal the Dream Collet and defeat Pretty Cure. To help, she must choose a team of four other students to be Pretty Cure so that she may help protect the Dream Collet and save Nuts from his entrapment within. Together with Cure Rouge, Cure Lemonade, Cure Mint and Cure Aqua, they form the Pretty Cure Team.

The Cure Rose Garden has always been concealed from human eye. Flora, the area's guardian, watches over it and ensures that the path to the secret area doesn't appear. However Eternal, an art organization known for amassing treasure and artwork from many worlds, is trying to get to the garden. To do this, they first invade the Palmier Kingdom and four other kingdoms. Flora senses the approaching a danger and sends a letter to the Pretty Cures. Their new mission is to find the four rulers that have fled from their kingdoms since they hold the Rose Garden Keys. To perform this mission, Nozomi and the others regain their ability to transform into PreCure Five. Yes. PreCure 5 GoGo. features the debut of Milk's human form, Kurumi Mimino and her Pretty Cure ego, Milky Rose.