In the sequel, a college student named Shouta Tsuikimiya moves into the house where his archaeologist father, Yuzo, had last been living when he disappeared. Shouta inadvertently awakens Doji-chan, a novice youkai hunter, when he finds her breastplate half-buried in the garden of the house. As if juggling his classes with dealing with the clingy and doting Doji-chan were not enough, Shouta soon finds that Doji-chan's fellow apprentices, as well as their mentor (Dogu-chan from the previous series), have taken up residence in his house as well.

In October 2010, Dogoo Girl premiered its sequel The Ancient Dogoo Girls (Kodai Shoujotai Doguun Faibu, "Ancient Girl Squad Dogoon 5"). The show adds five more Dogoo Girls portrayed by Misaki Momose, Rina Takeda, Manami Nomoto (of Idoling!!!), Maria Yoshikawa, and Haruka Dan. The theme song for the sequel is "Bakuha Seyo. Dogoon V" (Bakuha Seyo. Doguun Faibu, "It's a Blast. Dogoon V").