Spider-Man must stop the Sinister Syndicate from using an anti-gravity ray on New York, as well as rescue a group of reporters from them.

The ride turns park guests into last minute reporters of the Daily Bugle, and after boarding a vehicle known as the "Scoop", the rider learns that the Sinister Syndicate has captured the Statue of Liberty with an anti-gravity gun. This evil group is made up of five famous Spider-Man villains; Doctor Octopus, Scream, Electro, Hydro-Man, and the Hobgoblin, and it is up to Spider-Man to defeat the villains and save the guests.