The adventures of average suburban boy Johnny Test, who wittingly plays test subject for his genius twin sisters' various experiments, reluctantly fighting evil villains in the process.

Johnny Test features special guest stars in the series have included Lance Bass, Fred Willard, Penn Jilette, Jane Lynch, Jon Polito, Brian George, Snoop Dogg, James Belushi, Kathy Najimy, Ice-T, Dale Earnhardt Jr. and Bob Glouberman. Every episode contains 2 11-minute story segments and 2 segments including "May I Have a Word?" and "Silly Songs with Johnny".—Kids WB!