CSI spin off which follows an elite group of Crime Scene Investigators (CSI's) from the Miami-Dade Police Department (MDPD), who use advanced forensic science and methods to solve complex crimes in cross-cultural Miami, Florida.

Horatio Caine (David Caruso), a former homicide detective, heads a group of investigators who work crimes amid the steamy, tropical surroundings and cultural crossroads of Miami. His team includes Calleigh Duquesne (Emily Procter), a bilingual Southern beauty with a specialty in ballistics; Lieutenant Megan Donner (Kim Delaney), Detective Eric Delko (Adam Rodriguez), an underwater recovery expert; Walter Simmons (Omar Benson Miller), a detective who forces the "CSIs to do more science and research instead of relying on databases", Los Angeles Police transfer Jesse Cardoza (Eddie Cibrian), former FBI agent Natalia Boa Vista (Eva LaRue), and Ryan Wolfe (Jonathan Togo), a master of genetics recruited following the death of Detective Timothy Speedle (Rory Cochrane). The team are assisted by Coroner Alexx Woods (Khandi Alexander), who began her career as a medical Examiner in New York, and her replacement Tara Price (Megalyn Echikunwoke) Miami Dade Police Sergeant Frank Tripp (Rex Linn), and Horatio's sister-in-law, Detective Yelina Salas (Sofia Milos) both of whom act as the team's legal muscle. During their investigations, the team cooperate with both allies and nemeses, including Internal Affairs Lieutenant Rick Stetler (David Lee Smith), States Attorney Rebecca Nevins (Christina Chang), Medical Examiner Tom Loman (Christian Clemenson), and newly minted detective Sam Owens (Taylor Cole). Together, these investigators collect and analyze the evidence to solve the crimes and to vindicate those who often cannot speak for themselves - the victims.