- Charlie Adler
- Silverbolt / Guardian Prime (voice)
- Jack Angel
- Ultra Magnus / Astrotrain / Ramjet / Sentinel Prime / Quintesson Judge #3 / Sweep (voice)
- Arlene Banas
- Carly Witwicky
- Michael Bell
- Scrapper / Primon (voice)
- Gregg Berger
- Grimlock / Long Haul (voice)
- Susan Blu
- Arcee / Marissa Fairborne / Woman (voice)
- Corey Burton
- Spike Witwicky / Prime Nova (voice)
- Roger C. Carmel
- Cyclonus / Motormaster / Quintesson Judge #2 / Unicron (voice)
- Victor Caroli
- Narrator
- Philip L. Clarke
- Dead End / Tantrum / Zeta Prime / Abdul Fakkadi (voice)
- Regis Cordic
- Menasor / Quintesson Judge #1 / Judge Deliberata / Sharkticon (voice) (as Rege Cordic)
- Peter Cullen
- Optimus Prime
- Bud Davis
- Metroplex / Predaking / Dirge (voice)
- Paul Eiding
- Perceptor
- Ron Gans
- Drag Strip
- Brad Garrett
- Trypticon
- Dick Gautier
- Rodimus Prime / Sweep (voice)
- Ed Gilbert
- Blitzwing / Trooper Sullivan / Man (voice)
- Johnny Haymer
- Swindle / Vortex (voice)
- Milton James
- Blast Off
- Buster Jones
- Blaster
- Aron Kincaid
- Sky Lynx
- Joe Leahy
- Razorclaw
- Jeff MacKay
- Fireflight
- David Mendenhall
- Daniel Witwicky
- John Moschitta Jr.
- Blurr
- Alan Oppenheimer
- Warpath / Breakdown (voice)
- Rob Paulsen
- Slingshot
- Tony Pope
- Wreck-Gar
- Hal Rayle
- Pipes / Skuxxoid / Sweep (voice)
- Neil Ross
- Springer / Bonecrusher / Announcer (voice)
- Ted Schwartz
- Tailgate
- John Stephenson
- Kup / Outpost Officer (voice)
- Frank Welker
- Galvatron / Wheelie / Soundwave / Laserbeak / Rumble / Mixmaster / Sharkticon / Sweep / Computer (voice)
