- Aiden Turner
- Miles Cambridge
- Kelly Greyson
- Jocelyn Stewart
- Christopher Judge
- Sampson
- Rusty Joiner
- John Rolfe
- Travis Willingham
- Lord Carnal
- Laura Bailey
- Jane Pierce
- Jim McKeny
- Lord Admiral
- Joanie Stewart
- Sarah
- Jon Monastero
- Dr. Borgetti
- Mark Dacascos
- Nantauquas
- Peter Spencer
- Jeremy Sparrow
- Paul Ganus
- Pirate Adam Crampton
- John Neely
- Pirate James Riley
- Jefferson Cox
- Pirate George Smythe
- Thurman Bryan
- Pirate Jack O'Bannon
- Randall Archer
- Pirate #1
- Brian Avery
- Pirate #2
- David Brian Martin
- Pirate #3
- Matt Bengston
- Pirate #4
- James Cawley
- Pirate #5
- David Doersch
- Michael Wright
- E. Parker Webb
- Ruffian #1
- Scott Ludeke
- Marshall
- Joseph Gray
- Leftenant
- Michael Bayouth
- Jamestown Jailer
- Chris Durand
- Prince Royal Mate
- Kassi Crews
- Matron
- Tom Trigo
- Spanish Galley Chief
- Paul Burke
- River Pirate Captain
- Tony Wade
- Chief Opechancanough's Son
- Erika Castillo
- Pocahontas
- John A. Lorenz
- Chief Opechancanough
- Eric Varnell
- Dandy on the Dock
- CJ Legare
- Elizabeth Chilton
- Nicholas Martin
- Powder Keg Boy
- Kera O'Bryon
- Agreeable Maiden
- Andrew M. Price
- Tall Lad
- Vince Rodriguez
- Spanish First Mate
- Emelie Jeffries
- Native Woman #1
- Lenecia Renée Porro
- Native Woman #2
- Vanessa Bolin Clemens
- Native Woman #3
- Josiah Blount
- Slave
- Ron Campana
- Settler / Dandy
- Michael Carey
- Ruffian #3
- Carson Cash
- Royal Officer
- Paul D'Elia
- Pikeman
- Eddie Davenport
- River Pirate
- Chase Skylar DeMayo
- Pirate #6
- Gene Hansen
- Elder Member-House of Burgess
- Alexandra Hernly
- Jamestown Maiden
- Loubna Hoggard
- Native Woman #4
- Erin McShane
- Maiden
- Andrew Paterini
- Stand In for Rusty Joiner
- Andrew Ragan
- Personal Guard of the Governor
- John Rhys-Davies
- Governor Yeardly
- Zach Steffey
- Spanish Galley Guard #1
- Jake Swing
- Pikeman / English Soldier / British Soldier / Dandy / Indian
- Harrison Van Dorn
- Spanish soldier
- Luis Vázquez
- Native American Warrior
- Bailey West
- Maiden
- Andrew Scott Bell
- English Soldier
- David Brantley
- Spanish Soldier / English Settler (uncredited)
- Rick Craft
- Spanish Officer / English Soldier (uncredited)
- Jessica Dole
- Maiden
- Brian Elder
- Settler / British Soldier (uncredited)
- Lucas N. Hall
- Spanish Officer
- Wayne W. Johnson
- Native American Warrior
- James Judice
- Spanish Officer / Settler (uncredited)
- Ed Mantell
- Soldier
- Robert Messarge Jr.
- Spanish Officer
- Dennis Strawderman
- Captain of the Guard
- Gary Sundown
- Native American Warrior
- Brenda Van Dorn
- Maiden
- Nicholas Williamson
- Spanish Officer
- Yuriy Zubarev
- Field Soldier
