- Sylvia Sidney
- June Tolliver
- Henry Fonda
- Dave Tolliver
- Fred MacMurray
- Jack Hale
- Fred Stone
- Judd Tolliver
- Nigel Bruce
- Major Thurber
- Beulah Bondi
- Melissa Tolliver
- Robert Barrat
- Buck Falin
- George 'Spanky' McFarland
- Buddie Tolliver
- Fuzzy Knight
- Tater
- Otto Fries
- Corsey
- Samuel S. Hinds
- Sheriff
- Alan Baxter
- Clayt Tolliver
- Fern Emmett
- Lena Tolliver
- Richard Carle
- Ezra Tolliver
- Henry Brandon
- Wade Falin
- Jess Barker
- Merd Falin
- Bob Kortman
- Gorley Falin
- Frank Rice
- Zeke Denker
- Charles Middleton
- Blacksmith
- Clara Blandick
- Gaptown Landlady
- Irving Bacon
- Mailman
- John Larkin
- Ebony
- Merrill McCormick
- Falin Clan Member
- Ricca Allen
- Clayt's Wife
- Margaret Armstrong
- Ezra's Wife
- Charlene Arnold
- Undetermined Secondary Role
- Martin Beaumon
- Lumberjack
- John Beck
- Tolliver Clan Member
- Hank Bell
- Tolliver Clan Member
- James Burke
- Leader
- Fred Burns
- Tolliver Clan Member
- Powell Clayton
- Dave at age 5
- Jim Corey
- Falin Clan Member
- Jack Curtis
- Store Clerk
- Lowell Drew
- Bartender
- George Ernest
- Dave at age 10
- Betty Farrington
- Louisville Teacher
- Bud Geary
- Tolliver Clan Member
- Edward LeSaint
- Tolliver Clan Member
- Frank McGlynn Sr.
- Preacher
- Lee Phelps
- Taylor
- Russ Powell
- Storekeeper
- Tuffy
- The Dog
- Hilda Vaughn
- Gaptown Teacher
- Jim Welch
- Tolliver Clan Member
- Norman Willis
- Old Dave (in prologue)
- Charlotte Wynters
- Katherine Hale
