- Vera Drew
- Joker the Harlequin
- Lynn Downey
- Joker's Mother
- Kane Distler
- Mr. J
- Nathan Faustyn
- Penguin
- David Liebe Hart
- Ra's al Ghul
- Phil Braun
- Batsy
- Griffin Kramer
- Young Joker
- Christian Calloway
- Doctor Crane
- Trevor Drinkwater
- Edward Nygma aka The Riddler
- Ruin Carroll
- Ivy
- Tim Heidecker
- Perry White
- Denali Winter
- Clark
- Bambi Belle
- Lois
- Ember Knight
- Mxy
- Bob Odenkirk
- Bob the Goon
- Scott Aukerman
- Mister Frieze
- Alec Robbins
- Mr. Boop
- Sandy Honig
- Mr. Boop's Wife
- Dan Curry
- Bane
- Cassie Hamilton
- Nicole Kidman
- Jay Speedy Weingarten
- The Clown Who Gags
- Matthew Goldin
- Stiles Stilinski
- Eliot Glazer
- Lil Timmy Too Times
- Cricket Arrison
- Creeper
- Mia Moore Marchant
- Helena the Huntress
- Maria Bamford
- Lorne Michaels
- Robert Wuhl
- Self
- Austen Reeder
- Smylex Narrator
- Abby Clark
- Sad Wife
- Benjamin Morey
- Golfing Husband
- Dan Kypers
- UCB Orientation Narrator / Mad Hatter
- Jeremy Franchi
- Suicide Cop
- Ted Reidy
- Jimmy Olsen
- Daniella Baker
- Catwoman
- Lydia Wolfie
- Anarky / Harlequin Auditioner
- David Allen
- Suicide Cop's Partner
- Nateo Caballero
- UCB Receptionist
- Arden Hughes
- Audition System
- Harry McDonough
- UCB MC / Det. Harvey Bullock
- Rome Davis
- Therapist
- Jeremy Long
- Det. Melody McKenna
- Eleanor Cassady
- Hotline Operator
- Beatrice DiCicco
- Newscaster 1
- Emily Lynch
- Newscaster 2
- Michelle Stone
- Newscaster 3
- Ashe Pignataro
- Newscaster 4
- Calvin Dilibero
- Warmup Jokeman
- Zoë Limbrick
- Warmup Harlequin
- Jack Walker Nixon
- Ledger Jokeman
- Wylie Anderson
- Dini Harlequin 2
- Jake Noll
- Freddy
- Isabella Marano
- Dini Harlequin
