- Forrest Whaley
- Â Batman / ... 52 episodes, 2007-2024
- Keshen Sheken
- Â Alfred / ... 26 episodes, 2008-2023
- Brock Baker
- Â Announcer / ... 12 episodes, 2009-2019
- Mason Howerton
- Â Nightwing / ... 11 episodes, 2008-2019
- Kim Smith
- Â Barbara Gordon / ... 6 episodes, 2010-2019
- Alycia Marie
- Â Wonder Woman / ... 5 episodes, 2012-2022
- Alyssa Whaley
- Â Additional Voices / ... 5 episodes, 2019-2022
- Sean Willets
- Â Computer / ... 4 episodes, 2011-2019
- Toby Dale
- Â Flash 4 episodes, 2013-2019
- Michael J. Gallagher
- Â Riddler / ... 3 episodes, 2010
- Jonathan Aubrey
- Â Area 51 Soldier / ... 3 episodes, 2019
- Thomas Fyfe
- Â DJ / ... 2 episodes, 2009-2010
- DarthMilo77
- Â Mr. Freeze 2 episodes, 2009-2010
- Dave Smith
- Â Commissioner Gordon / ... 2 episodes, 2010
- Paul Fabrick
- Â Lex Luthor 2 episodes, 2013
- Chris Little
- Â Green Lantern 2 episodes, 2019
- Jake Blandina
- Â Additional Thug / ... 2 episodes, 2011-2012
- Patrick Girts
- Â Mark Hamill Joker / ... 2 episodes, 2011-2019
- Zach Macias
- Â Guy Getting Mugged / ... 2 episodes, 2010-2019
- Paul Hollingsworth
- Â Riddler / ... 2 episodes, 2013-2019
- Ego Slave
- Â Lil Birdy 1 episode, 2022
- Shane Dawson
- Â Spider-Man 1 episode, 2009
- DrunkenMonkey2
- Â Scarecrow 1 episode, 2009
- Jordan Haro
- Â Commissioner Gordon 1 episode, 2017
- Lloyd Ahlquist
- Â Donald Trump 1 episode, 2019
- Moe Rock
- Â Poison Ivy 1 episode, 2023
- WastedxLama
- Â Joker 1 episode, 2008
- Abigail Reed
- Â Iris West 1 episode, 2013
- Lord Frank
- Â Homophobic Prisoner 1 episode, 2023
- ShadowRocks321
- Â Irina Spalko 1 episode, 2008
- Brett Dixon
- Â McDonald's Guy 1 episode, 2010
- Amanda Teeter
- Â Street Slut 1 episode, 2010
- J.T. Hagaman
- Â Newsman 1 episode, 2009
- SwiftKarateChop
- Â Bane 1 episode, 2009
- Ksicsfaces
- Â Riddler 1 episode, 2009
- Jacob Cline
- Â Area 51 General 1 episode, 2019
- Darkman13Forever
- Â Alfred / ... 1 episode, 2008
- Shane Whaley
- Â Guest Voice 1 episode, 2010
- Stephen Brennan
- Â Additional Thug 1 episode, 2012
- Chris Little
- Â Guest Voice 1 episode, 2010
- Jason Steele
- Â Sharks 1 episode, 2013
- Noah Howerton
- Â Bad Carma Driver 1 episode, 2013
- Amanda Taylor
- Â Woman at Bar 1 episode, 2019
- Adam Hyatt
- Â Other Jokers 1 episode, 2019
- Bobby Burns
- Â Damien Wayne 1 episode
