- Scott McClure
- Â The Joker / ... 32 episodes, 2008-2017
- Steven Molony
- Â Dr. Jeremiah Arkham / ... 17 episodes, 2009-2017
- Kira Rudjen
- Â Dr. Harleen Quinzel 9 episodes, 2008-2010
- Kendra Cashmore
- Â Harleen Quinzel / ... 7 episodes, 2009-2014
- Paul Vonasek
- Â Lex Luthor / ... 7 episodes, 2009-2017
- Torrey Halverson
- Â Jonathan Crane / ... 6 episodes, 2009-2017
- Joy Dolo
- Â Pearl / ... 6 episodes, 2009-2014
- Patrick J. Thompson
- Â Lyle Bolton / ... 6 episodes, 2012-2017
- Andrew DeVary
- Â Ted / ... 4 episodes, 2009-2013
- Steve Gibbs
- Â Lyle Bolton 4 episodes, 2009-2010
- Katie Adducci
- Â Summer Gleason 4 episodes, 2010-2017
- Maddisyn Carter
- Â Catwoman / ... 4 episodes, 2012-2017
- Joseph T. Kramer
- Â Steve 3 episodes, 2008-2013
- Dan Glaser
- Â Dr. Cavendish 3 episodes, 2013-2017
- Lucas Vonasek
- Â Jack Ryder 3 episodes, 2013-2017
- Dayna Del Val
- Â Barbara Gordon 3 episodes, 2013-2017
- John Rian
- Â Harvey Bullock 2 episodes, 2009-2012
- John Ford-Dunker
- Â Jim Corrigan 2 episodes, 2009-2010
- Julian Weinstein
- Â Mac Rodello / ... 2 episodes, 2009-2010
- Nicolas Lee-Demetrius Korokidas
- Â Roman Sionis / ... 2 episodes, 2010-2014
- Matthew Pitner
- Â Guy Kopski 2 episodes, 2010-2013
- Al Odden
- Â Harold Quinzel 2 episodes, 2010-2013
- Maxwell Heesch
- Â Quincy Sharp 2 episodes, 2010
- Kelli Breslin
- Â Andrea Beaumont 2 episodes, 2013-2017
- Bill Dablow
- Â Rupert Thorne 2 episodes, 2013-2017
- Jim Alger
- Â Miles 'Matches' Malone 2 episodes, 2013-2014
- Chadwick Richard Anderson
- Â Harvey Dent / ... 2 episodes, 2014-2017
- Timothy J. Meyer
- Â Garrett Kopski 1 episode, 2010
- Jenna Halvorson
- Â Helen Strange 1 episode, 2010
- Andrea Donahue
- Â Wedding Guest 1 episode, 2010
- Michael Chappo
- Â Father McCale 1 episode, 2010
- Stephen Roise
- Â Wedding Guest 1 episode, 2010
- Christina Collins
- Â Wedding Guest 1 episode, 2010
- John Rian
- Â Harvey Bullock 1 episode, 2010
- Sebastian John Hull
- Â Jack Ryder 1 episode, 2010
- Ben Stommes
- Â Wedding Guest 1 episode, 2010
- Matt Berdahl
- Â Wedding Guest 1 episode, 2010
- Laura Posterick
- Â Wedding Guest 1 episode, 2010
- Peder Lysne
- Â Mike Engel 1 episode, 2010
- Brianna Lucas
- Â Pamela Isley 1 episode, 2010
- Maya Waterman
- Â Dr. Leslie Thompkins 1 episode, 2012
- Grant Ertl
- Â Alberto Falcone 1 episode, 2012
- Wade Everett
- Â Roland Daggett 1 episode, 2013
- Rachel Grubb
- Â Mercy 1 episode, 2013
- Nicholas Collins
- Â Roman Sionis 1 episode, 2013
- Parker Shook
- Â Halliwell 1 episode, 2013
