- Kari Alison Hodge
- Catwoman / Selina Kyle
- Taylor C. Baker
- Dr. Pamela Isley / Poison Ivy
- Giselle Bastanchury
- Dr. Harleen Quinzel / Harley Quinn
- Eric Eberle
- Dick Grayson / Robin
- Barbie Ellen
- Shauna Miles
- Kayla Fields
- Barbara Gordon / Batgirl
- Will Hariton
- Dick Grayson / Robin
- Derby Hart
- Gladys Miles
- Shamel Hashish
- Ra's Al Ghul
- Steven Honeycutt
- Narrator / Robby the Robot
- Jeffrey Johnson
- Mayor Linseed
- B.J. Lucas
- Bruce Wayne / Batman
- Evan William Miller
- Dr. Miles
- Diana Mai Nguyen
- Tatsu Yamashiro / Katana
- Zack Owens
- Narrator / Robby the Robot (voice)
- Ron L. Palmer
- The Joker
- Philip A. Pirio
- Commissioner James Gordon
- Francisco Rascon
- Bane
- Robby the Robot
- Self
- Christina Russo
- Violet
- Paul Ryder
- Alfred Pennyworth
- Isabel Sobrepera
- Gladys Miles
- Paula Tsoiasue
- Talia Al Ghul
- Steve VanErt
- Detective Harvey Bullock
