- Tom Gore
- Dr. Alex Ross
- Hurley Paul
- GCPD Officer
- Bill Mulligan
- Arkham Guard
- Crystal Cleveland
- Poison Ivy
- William R Frady
- Dr. Jonathan Crane / Scarecrow (as William Frady)
- Faneal Godbold
- Harley Quinn
- Rob Kellum
- Dr. Jeremiah Arkham
- James Hurley
- Aaron Cash
- Rick Rector
- Detective Eckhart
- Lucky Mangione
- Victor Zsasz
- Demond Alvin
- Arkham Security
- Sammie Cassell
- Harleen Quinzel's Father
- Jamaas Britton
- Arkham Guard
- Chuck Sloan
- Harvey Bullock
- Gary Williams
- Arkham Guard
- Daniel Allen Dorn
- Security Guard / Black Mask / Victim
- Brice Cochran
- Barry Quinzel
- Judy Dishmond
- Arkham Nurse
- Brian Phillips
- The Riddler
- Kyle King
- Gangster Corpse
- Brittany Buell
- Margot the Call Girl
- Destiny Rector
- Arkham Nurse
- Christopher Polston
- Guard
- Joshua Perkins
- Arkham Guard
- Natisha Vest
- Firefly
- Jessie Rivera
- Coach
- David Dubois
- Arkham Guard
- Jatanna Willis
- Arkham Nurse
- Aspen Gilbert
- Arkham Guard
- William Frady
- Dr. Jonathan Crane / Scarecrow
- Matthew Law
- Arkham Guard / Joker's Goon
- D'Erricka Byers
- Dr. Arkham's Assistant
- Melquinn Redfern
- College Student
- Jonathan Barber Jr.
- Joker
- Racquel Rogers
- Nurse
- Charles Strickland
- Gotham Police Officer
- Elise Sherman
- College Student
- Beck Lippel
- Hush
- Sonya Parker
- Arkham Nurse
- John C. Martin
- Bruce Wayne / Batman
- Melissa Rector
- Professor
- Dylan Carpenter
- Inmate
- Gene Perry
- Gangster Corpse
- Pat Mninski
- Arkham Guard
- Ashley McHam
- Barbara Gordon
- Rob Cochran
- Killer Croc
- Devin DePalma
- Young Bruce Wayne
- William Fripp
- Dr. Gerald Crane
- Sai Johnson
- Punchline
- Clam Sepulveda
- Professor Helton
- Jonathan Willis
- Arkham Guard
- Shon Johnson
- Deathstroke
- Jim Sherman
- Professor
- Heather Six
- Selina Kyle / Catwoman
- Gregbo Watson
- Arkham Thug
- Patrick Greene
- Gangster Corpse
- Joeseph Moose
- Arkham Guard
- Brooklyn Flamm
- Magpie
- Shannon Jay
- Gotham Officer
- Eric Alkins
- Joker Victim
- Lillian Barber
- Young Harleen Quinzel
- Panzyta Kenan
- Security Guard
- Hunter Diamond
- College Student
- Daphne Reeder
- Security Guard
- Ann Phillips
- Talia al Ghul
- Misty Yates
- Laura Bailey
- Brandi Cochran
- Harleen's Mother
- Mackenzie Payne
- Arkham Nurse
- Emma Polston
- Arkham Nurse
- Zach Lutz
- Guard
- Kenneth Walker
- Joker Victim
- David Gardner
- Arkham Guard
- Ariana Henry
- Corpse
- Shanique Sumter
- College Student
- Mark Lavecc
- Gangster Corpse
