- Andrew S. Bates
- Peter Parker / Spider-Man (voice)
- Jean V. Alexander
- Aunt May Parker
- September Day
- Mary Jane Watson
- Bob Carter
- Green Goblin / Sandman / Kraven the Hunter / Captain America / J. Jonah Jameson (voice)
- Jason Elkins
- Johnny Storm / Human Torch / Electro (voice)
- Tony Messano
- Vulture / Uncle Ben Parker (voice)
- E.J. Smith
- Nick Fury
- Lacey Patten
- Gwen Stacy
- Sally Rose Bates
- Doris
- Matt Strach
- Bobby Drake / Iceman (voice)
