- Lillian Gish
- Elsie - Stoneman's Daughter
- Mae Marsh
- Flora Cameron - The Pet Sister
- Henry B. Walthall
- Col. Ben Cameron aka The Little Colonel
- Miriam Cooper
- Margaret Cameron - The Elder Sister
- Mary Alden
- Lydia Brown - Stoneman's Mulatto Housekeeper
- Ralph Lewis
- Hon. Austin Stoneman - Leader of the House
- George Siegmann
- Silas Lynch - Mulatto Lieut. Governor
- Walter Long
- Gus - A Renegade Negro
- Robert Harron
- Tod - Stoneman's Younger Son
- Wallace Reid
- Jeff - The Blacksmith
- Joseph Henabery
- Abraham Lincoln
- Elmer Clifton
- Phil - Stoneman's Elder Son
- Josephine Crowell
- Mrs. Cameron
- Spottiswoode Aitken
- Dr. Cameron
- George Beranger
- Wade Cameron - The Second Son
- Maxfield Stanley
- Duke Cameron - The Youngest Son
- Jennie Lee
- Mammy - The Faithful Servant
- Donald Crisp
- Gen. Ulysses S. Grant
- Howard Gaye
- Gen. Robert E. Lee
- Bennie Billings
- Child
- Monte Blue
- Minor Role
- Harry Braham
- Cameron's Male Servant
- Beulah Burns
- Sick woman's child
- Bob Burns
- Klan Leader
- Edmund Burns
- Klansman
- Edward Burns
- Klansman
- Fred Burns
- Klansman
- Thelma Burns
- Sick Woman's Child
- David Butler
- Northern Soldier / Confederate Soldier (uncredited)
- Peggy Cartwright
- Young Girl in Cabin
- William E. Cassidy
- Minor Role
- Dark Cloud
- General at Appomatox Surrender
- Lenore Cooper
- Elsie's Maid
- Sam De Grasse
- Senator Charles Sumner
- William De Vaull
- Nelse - An Old-Fashioned Negro
- Charles Eagle Eye
- Man Who Falls from Roof
- John Ford
- Klansman on Horse Holding Up Hood with Hand
- Alberta Franklin
- Minor Role
- William Freeman
- Jake / Sentry at Hospital (uncredited)
- Gibson Gowland
- Undetermined Secondary Role
- Olga Grey
- Laura Keene
- D.W. Griffith
- Self (1931 reissue version)
- Frederic Hamen
- Minor Role
- Russell Hicks
- Minor Role
- Walter Huston
- Walter Huston (1930 re-release promotion)
- Alberta Lee
- Mrs. Lincoln
- Elmo Lincoln
- White-Arm Joe - Blacksmith
- Betty Marsh
- Child with Dr. Cameron
- Donna Montran
- Belle of 1861
- Eugene Pallette
- Union Soldier
- Vester Pegg
- Minor Role
- Jack Pickford
- Negro
- Hayes E. Robertson
- Man Going Inside While Ku Klux Klan is Watching
- Alma Rubens
- Belle of 1861
- Allan Sears
- Klansman
- Charles Stevens
- Volunteer Who Reports Piedmont Raid
- Madame Sul-Te-Wan
- Black Woman - Dr. Cameron's Taunter
- Erich von Stroheim
- Man Who Falls From Roof
- George Walsh
- Minor Role
- Raoul Walsh
- John Wilkes Booth
- Jules White
- Confederate Soldier
- Violet Wilkey
- Flora Cameron as a Child
- Tom Wilson
- Stoneman's Servant
- Mary Wynn
- Minor Role
