- Sander Schwartz
- Self - President of Warner Bros. Animation
- Michael Goguen
- Self - Supervising Producer
- Duane Capizzi
- Self - Supervising Producer & Story Editor
- Jeff Matsuda
- Self - Producer & Art Director
- Danielle Judovits
- Self / Barbara Gordon / Batgirl
- Dan Castellaneta
- Scarface
- Piera Coppola
- Poison Ivy
- Alastair Duncan
- Alfred Pennyworth
- Will Friedle
- Gearhead
- Tom Kenny
- The Penguin
- Mitch Pileggi
- Commissioner Gordon
- Rino Romano
- Batman
