- Tommy Kelly
- Tom Sawyer
- May Robson
- Aunt Polly
- Walter Brennan
- Muff Potter
- Victor Jory
- Injun Joe
- David Holt
- Sid Sawyer
- Jackie Moran
- Huckleberry Finn
- Nana Bryant
- Mrs. Thatcher
- Olin Howland
- Mr. Dobbins - Schoolmaster
- Donald Meek
- Mr. Walters- Sunday School Superintendent
- Charles Richman
- Judge Thatcher
- Victor Kilian
- Sheriff
- Marcia Mae Jones
- Mary Sawyer
- Mickey Rentschler
- Joe Harper
- Cora Sue Collins
- Amy Lawrence
- Philip Hurlic
- Little Jim
- Ann Gillis
- Becky Thatcher
- Margaret Hamilton
- Mrs. Harper
- Eric Alden
- Assistant Defense Attorney
- Erville Alderson
- District Attorney
- Donnie Allen
- Schoolboy
- Bobbie Allert
- Schoolgirl
- Byron Armstrong
- Willy Fisher
- Joanne Arnold
- Schoolgirl
- Arthur Aylesworth
- Defense Attorney
- Buddy Bange
- Abner
- Frank Bellan
- Schoolboy
- Georgie Billings
- Benjamin Rogers
- Tom Braunger
- Schoolboy
- Louis Brenn
- Schoolboy
- Tommy Bupp
- Schoolboy
- George Bush
- Man
- Jackie Butler
- Schoolboy
- Spring Byington
- Widow Douglas
- Connie Cezon
- Schoolgirl
- Hugh Chapman
- Schoolboy
- Reba Churchill
- Schoolgirl
- Dolly Conlin
- Schoolgirl
- Luke Cosgrave
- Old Soldier
- Joy Cowden
- Schoolgirl
- Patsy Currier
- Schoolgirl
- Frank Darien
- Storekeeper
- Dix Davis
- Schoolboy
- Tim Davis
- Schoolboy
- Shirley Doble
- Schoolgirl
- Dee Dodd
- Schoolboy
- Roland Drew
- Dr. Robinson
- Frank Elmquist
- Schoolboy
- Charles Flickinger
- Schoolgirl
- Betsy Gay
- Susie Harper
- June Renee Gertler
- Schoolgirl
- Kenneth Grant
- Schoolboy
- Audrey Halligan
- Schoolgirl
- Sally Harper
- Schoolgirl
- Louis Herbers
- Schoolboy
- Phyllis Holt
- Schoolgirl
- Junior Hughes
- Schoolboy
- Ronald Hughson
- Schoolboy
- Malcolm Hutton
- Schoolgirl
- Hollis Jewell
- Schoolboy
- Cammilla Johnson
- Little Girl
- Seessel Anne Johnson
- Little Girl
- Sharon Keller
- Schoolgirl
- Margaret Kerry
- Schoolgirl
- Evelyn Kurt
- Schoolgirl
- Leonard Lawrence
- Schoolboy
- Edward LeSaint
- Coroner at Trial
- Faith Lieberson
- Schoolgirl
- Fern Lieberson
- Schoolgirl
- Frances Lieberson
- Schoolgirl
- Jack Lindquist
- Schoolboy
- Buddy Londelius
- (uncredited)
- Joan Lott
- Schoolgirl
- Herbert MacGregor
- Schoolboy
- Marilyn Marlin
- Schoolgirl
- Lon McCallister
- Schoolboy
- Leona McDowell
- Schoolgirl
- Frank McGlynn Sr.
- Minister
- Robert McKenzie
- River Authority
- Vivian McLean
- Schoolgirl
- Elaine Merk
- Schoolgirl
- Dickie Meyers
- Schoolboy
- Jackie Cagney Miller
- Schoolgirl
- Bobby Misrach
- Schoolboy
- Evelyn Misrach
- Schoolgirl
- Bruce Mitchell
- Deputy
- Jack Morris
- Schoolboy
- Betty Ann Muir
- Schoolgirl
- June Muir
- Schoolgirl
- Bob Murphy
- Bailiff
- Harry Myers
- Irate Churchgoer
- Jeanette Noeson
- Schoolgirl
- Frank O'Connor
- Mr. Harper
- June Parkes
- Schoolgirl
- Mary Elizabeth Perkins
- Schoolgirl
- Jean Porter
- Pauline - Schoolgirl
- Marjorie Prax
- Schoolgirl
- Spencer Quinn
- Schoolboy
- Bobby Scott
- Schoolboy
- Billy Shearer
- Schoolboy
- Arnold Statz
- Schoolboy
- Donn Stokes
- Schoolboy
- A.W. Sweatt
- Schoolboy
- Charles Unger
- Schoolboy
- Dan Wolheim
- Deputy
- Jimmy Zahner
- Boy
