- Mickey Rooney
- Huckleberry Finn
- Walter Connolly
- The 'King'
- William Frawley
- The 'Duke'
- Rex Ingram
- Jim
- Lynne Carver
- Mary Jane
- Jo Ann Sayers
- Susan
- Minor Watson
- Capt. Brandy
- Elisabeth Risdon
- Widow Douglass
- Victor Kilian
- 'Pap' Finn
- Clara Blandick
- Miss Watson
- Erville Alderson
- Sheriff at Jail
- Arthur Aylesworth
- River Queen Pilot
- Irving Bacon
- Tad
- Wade Boteler
- 1st Riverboat Captain
- Ed Brady
- Man at Show
- Harlan Briggs
- Mr. Rucker
- Nora Cecil
- Mrs. Shackleford
- Harry Cording
- Watermelon Thief
- Frank Darien
- Old Jailer
- Sarah Edwards
- Mrs. Annie Rucker
- Jesse Graves
- Wilkes' Black Butler
- George Guhl
- River Queen Engineer
- Roger Imhof
- Judge Logan
- Robert Emmett Keane
- Mr. Barklett
- Lew Kelly
- 1st Riverboat Pilot
- Leni Lynn
- Schoolgirl
- Anne O'Neal
- Miss Bartlett
- Sarah Padden
- Woman Wanting Needle Threaded
- Alonzo Price
- Deputy
- Sharon Randall
- Mary Adams
- Mickey Rentschler
- Harry
- Johnny Walsh
- Sam
- E. Alyn Warren
- Mr. Shackleford
- Billy Watson
- Eliot
- Delmar Watson
- Joe
- Harry Watson
- Ben Donaldson
