- Vincent Price
- Dr. Anton Phibes
- Joseph Cotten
- Dr. Vesalius
- Virginia North
- Vulnavia
- Terry-Thomas
- Dr. Longstreet
- Sean Bury
- Lem Vesalius
- Susan Travers
- Nurse Allen
- David Hutcheson
- Dr. Hedgepath
- Edward Burnham
- Dr. Dunwoody
- Alex Scott
- Dr. Hargreaves
- Peter Gilmore
- Dr. Kitaj
- Maurice Kaufmann
- Dr. Whitcombe
- Peter Jeffrey
- Detective Inspector Trout
- Derek Godfrey
- Crow
- Norman Jones
- Sgt. Tom Schenley
- John Cater
- Superintendent Waverley
- Alan Zipson
- 1st Police Official
- Dallas Adams
- 2nd Police Official
- James Grout
- Sergeant
- Alister Williamson
- 1st Policeman
- Thomas Heathcote
- 2nd Policeman
- Ian Marter
- 3rd Policeman
- Julian Grant
- 4th Policeman
- Hugh Griffith
- Rabbi
- Aubrey Woods
- Goldsmith
- John Laurie
- Darrow
- Barbara Keogh
- Mrs. Frawley
- Charles Farrell
- Chauffeur
- John Franklyn
- Graveyard Attendant
- Walter Horsbrugh
- Butler Ross
- Joanna Lumley
- Laboratory Assistant
- Mabel Etherington
- Masked Party Guest
- Ron Gregory
- Policeman Guarding Nurse Allen's Door
- George Hilsdon
- Police Photographer
- Juba Kennerley
- Gentlemens Club Member Calling For Quiet
- Julie Mendez
- Belly Dancer on Movie Screen
- Caroline Munro
- Victoria Regina Phibes
- Guy Standeven
- Morgan
- John Tatham
- Porter
- Robert Vossler
- Plainclothes Policeman
- Pearl Walters
- Masked Party Guest
- Tommy Windward
- Policeman
