- Moe Howard
- Moe
- Larry Fine
- Larry
- Curly Howard
- Curly
- Paul Winchell
- Self - Ventriloquist
- Jerry Mahoney
- Self - Ventriloquist's Dummy
- Knucklehead Smiff
- Self - Ventriloquist's Dummy
- Joe Bolton
- Officer Joe
- The Marquis Chimps
- Themselves - Trained Chimps
- Beatrice Blinn
- Nurse
- Stanley Blystone
- Longhorn Pete
- Symona Boniface
- Mrs. Bixby / Party Guest (archive footage) (uncredited)
- Lynton Brent
- Mr. Allen Radio Exec / Dognapper (archive footage) (uncredited)
- Edmund Cobb
- Construction Foreman
- Chester Conklin
- Pianist at Party
- Gino Corrado
- Italian Singer ("Micro-Phonies")
- Beatrice Curtis
- Mrs. Bedford
- Vernon Dent
- Mr.Blake / Marshall Boring (archive footage) (uncredited)
- Dudley Dickerson
- Cook / Roadside Worker (archive footage) (uncredited)
- Helen Dickson
- Party Guest
- Sam Flint
- Mr. Van Doren, Alice's Father
- Bess Flowers
- Party Guest / Mrs.Hadley (archive footage) (uncredited)
- June Foray
- Cinderella
- Joyce Gardner
- Little Jimmy Collins
- Julie Gibson
- Mrs. Collins
- Bud Jamison
- Acme Station Owner / Pete, Motorcycle Cop (archive footage) (uncredited)
- Johnny Kascier
- Thug
- Fred Kelsey
- The Boy's Boss
- Ethelreda Leopold
- Nurse
- Theodore Lorch
- General Muster / Prof. Sedletz (archive footage) (uncredited)
- Judy Malcolm
- Radio Station Receptionist
- Christine McIntyre
- Alice Andrews (Van Doren)
- Dorothy Moore
- Nurse
- Robin Raymond
- Nurse
- Alan Reed
- Prince
- Cy Schindell
- Workman / Attendant / Henchman (archive footage) (uncredited)
- Emil Sitka
- Sapington the Butler
- Barbara Slater
- Miss Lulu Quackenbush
- Clarence Straight
- Sam Collins, Motorcycle Cop
- Libby Taylor
- Mrs. Bedford's Maid
- Al Thompson
- Professor / Party Guest (archive footage) (uncredited)
- Victor Travis
- Party Guest
- John Tyrrell
- Shamus O'Brien / Judge Hadley (archive footage) (uncredited)
- Duke York
- Kelly
