- David Figueroa
- Peter Parker / Spider-Man (voice)
- Paul Hikari
- Norman Osborn / Green Goblin (voice)
- Kim Khan
- Gwen Stacy
- Kevin Liberty
- Wilson Fisk
- Alejandro Fletes
- Daredevil / Matt Murdock (voice)
- Dane Liberatore
- Harry Osborn
- D. Tyler Fultz
- J. Jonah Jameson
- Majingar
- George Stacy
- Stephen Phillip
- Slyde
- Jessie Yun
- Aunt May
- Shaun Brackett
- Hoffman / Mysterio (voice)
- PhoenixDiamonite
- Betty Brandt
- Dontell McMichael
- Robbie Robertson
- Jerry Flowers III
- Flash Thompson
- Lance Newman
- James Wesley
- Will Evans
- Thug #1 / Police Officer #1 (voice)
- Paige Martin
- Helen Stacy
- Rhys Griffiths
- Thug #2 / OSCORP Employee (voice)
- Samuel Parish
- Thug #3
- Evan Newburn
- Thug #4
- Nick Evans
- Police Officer #3 / William Harris (voice)
- Hiletman
- Police Officer #2
- Tossler
- Police Officer #4
- Ronald Melton Braxton
- Fisk Henchman #1
- Fasttman9090
- Fisk Henchman #2
- Tempest Rogers
- Fisk Henchman #3
- Brady Hendricks
- OSCORP Security Guard / Fisk Guard #2 (voice)
- Timothy Rowland
- Fisk Driver
- Ray Si
- Thief / OSCORP Scientist #1 (voice)
- Braigar
- Fisk Guard #1
- Huntylicious
- Fisk Guard #3
- Lewis Talbot
- Fisk Guard #4
- Crow Silkfeather
- OSCORP Scientist #2
