- James Avery
- Jim Rhodes / War Machine (voice)
- Sara Ballantine
- Mary Jane Watson
- John Beard
- Newscaster
- Roscoe Lee Browne
- Kingpin
- Joseph Campanella
- Dr. Curt Conners
- Scott Cleverdon
- Cletus Kasady / Carnage (voice)
- Rebecca Kyler Downs
- Additional Voices
- Linda Gary
- Aunt May Parker
- Liz Georges
- Debra Whitman
- Ed Gilbert
- Dormammu
- Barbara Goodson
- Dr. Ashley Kafka
- Jennifer Hale
- Felicia Hardy
- Robert Hays
- Tony Stark / Iron Man (voice)
- Michael Horton
- John Jameson
- Tony Jay
- Baron Mordo
- Joan Lee
- Madame web
- Dawnn Lewis
- Detective Terri Lee
- Anthony Marciona
- Additional Voices
- Rodney Saulsberry
- Robbie Robertson
- Don Stark
- Rhino
