- David Figueroa
- Spider-Man / Peter Parker (voice)
- ShadowGamingVA
- Gwen Stacy
- Alex Newcomb
- The Lizard / Curt Connors (voice)
- Tarquin Irusa
- Hobgoblin / Harry Osborn (voice)
- Rachel Leon
- Aunt May
- Phoenix Artisan
- George Stacy
- Alejandro Fletes
- Daredevil / Matt Murdock (voice)
- Bobby B.
- Bernard
- D. Tyler Fultz
- J. Jonah Jameson
- Katie Otten
- Helen Stacy
- Jay Silver
- Bullseye
- Diamond Jo
- Betty Brant
- Dontell McMichael
- Robbie Robertson
- Shaun Brackett
- Hoffman
- Ishva
- Martha Connors
- Hamuel
- Billy Connors
- Brady Hendricks
- Eddie Brock
- Neal Aron
- The Shocker
- Kevin Liberty
- Wilson Fisk
- Michi Rampha
- Mary Jane Watson
- Paul Hikari
- Norman Osborn
- Dexter Manning
- Temporary Police Chief
- Cosmic Comfort
- Michael Badilino
- Biblical Blacksmith
- News Reporter
- Yenny Belles
- Harry's Assistant
- M Sheep
- Phil Watson
- Jak Wilmot
- Uncle Ben
- Ella Crium
- News Stand Owner / Additional Voices (voice)
- Keybladesonic
- Dealer
- Bryce Dahle
- Squad Leader
- Alexander David Matthews
- Foreman
- Avni Xaxa
- Oscorp Receptionist
- Kamskii
- Additional Voices
- Mrp4nc435
- Additional Voices
- Gabriel Lancaster
- Additional Voices
- Larsvoices
- Additional Voices
- Nubu Art
- Additional Voices
- Blake Swift
- Additional Voices
- Billy Vargus
- Additional Voices
- Kal
- Additional Voices
- Poolnoodlex
- Additional Voices
- Sigma
- Additional Voices
- Stephen Duerr
- Additional Voices
