- Robert Wisdom
- Alpha
- Tanc Sade
- Beta
- Patton Oswalt
- Fred
- Simon Baker
- Roderick Blank
- Mindy Cohn
- Trixie
- Winona Ryder
- Death Nell / Gillian De Raisx (#101)
- Keram Malicki-Sánchez
- Master Bitchslap
- Julie Bowen
- Fiona Wormwood (#29)
- Neil Flynn
- Zack
- Corinne Reilly
- Lizzie
- Cindy Pickett
- Roderick's Mother
- Winter Ave Zoli
- Alexis De Large (#37)
- Dash Mihok
- Lester
- Bryan Okes
- Buzzkilling Bodyguard
- Jessica Kiper
- Precious / Carlotta Valdes (#30)
- Bryan Ross
- Bachelor Party Buddy
- Greg Travis
- Vulgar Newsstand Vendor
- Sophie Monk
- Cynthia Rose (#31)
- David Magidoff
- Video Store Geek
- Marshall Bell
- Victor Rose III
- Buckley Norris
- Club Owner
- Amanda Walsh
- Stewardess Kathleen
- Candice Coke
- Greta Samsa (#32)
- Tarek Bishara
- Malcolm the Bartender
- William Caploe
- Barista Bill
- Zachary Gordon
- Barbecue Brat
- Rob Benedict
- Bow-Tie Bob
- Natassia Malthe
- Bambi (#63)
- Pollyanna McIntosh
- Thumper (#64)
- Leslie Bibb
- Dr. Miranda Storm
- Patrick Leo Holland
- Wonderful Waiter
- Ryan Cutrona
- Officer Krupke
- Nicole Bilderback
- Dr. Mirabella Stone (#66)
- Cean Okada
- Edwina Massey (#67)
- Siobhan Flynn
- Esther Fenchel
- Christopher Stapleton
- Terry Fenchel (#71)
- Lauren Hill
- Amberette
- Frances Fisher
- Hope Hartlight (#80)
- Retta
- Ethel Walters (#100)
- David Bortolucci
- Agent
- Cate Ferguson
- Death Nell Wannabee
- Annie Kehoe
- Death Nell Wannabee Two
- Skyler James Sandak
- Roderick Blank Jr.
- Matt Baker
- Rejected Clubgoer
- Elena Nikitina Bick
- Death
- Kacie Borrowman
- Vixen Blitzen (#69)
- Yara Brighton
- Death Nell Wannabe
- Laura Brokaw
- Julie Kohler
- Colette Claire
- School Girl
- Ingrid Coree
- Waitress
- Cole Downing
- Nerd
- Kevin Elms
- Fraternity President
- Aaron Geller
- Death Nell's First Victim
- Marcus Alexander Hart
- Comic Store Geek
- Ashley McCarthy
- Mona Farlow
- Marshall McClean
- Party Guy
- Jonas Neal
- Frat Guy
- Tony Nowicki
- Frat Guy
- Elisha Skorman
- Helena Hartford
- Heather Tocquigny
- Catholic School Girl
- Indira Varma
- Devon Sever
- Vitaliy Versace
- Waiter
