- Rod Taylor
- Sir Francis Drake
- Keith Michell
- Malcolm Marsh
- Edy Vessel
- Arabella Ducleau
- Terence Hill
- Babington
- Basil Dignam
- Sir Francis Walsingham
- Anthony Dawson
- Lord Burleigh
- Gianni Cajafa
- Tom Moon
- Irene Worth
- Queen Elizabeth I
- Arturo Dominici
- Don Bernardino de Mendoza, the Spanish Ambassador
- Marco Guglielmi
- Fletcher
- Esmeralda Ruspoli
- Mary of Scotland
- Rossella D'Aquino
- Potato
- Umberto Raho
- King Philip of Spain
- Aldo Bufi Landi
- Vigeois
- Giuseppe Abbrescia
- Chester
- Tony Casale
- Guard
- Luciana Gilli
- Indian Wife
- Rodolfo Lodi
- Courtier
- Mauro Mannatrizio
- Spanish Assassin / Drake's Crew (uncredited)
- Luciano Melani
- Captain Winter
- Franco Pasquetto
- Spanish Assassini / Drake's Crew (uncredited)
- Attilio Pelegatti
- Conspirator
- Mariano Peroni
- Courtier
- Massimo Righi
- Lord of the Royal Court
- Anna Santarsiero
- Indian Wife
- Gianni Solaro
- Admiral Medina Sedonia
- Jacopo Tecchi
- Garcia
- Bruno Ukmar
- Emmanuel
- Franco Ukmar
- Francisco
- Giovanni Ukmar
- Drake's Crew
- Sergio Ukmar
- Drake's Crew
- Adriano Vitale
- Recalde
- Alfredo Zammi
- English Soldier
