- Caroll Spinney
- Big Bird / Oscar the Grouch (voice)
- Frank Oz
- (voice) (archive footage)
- Jerry Nelson
- Count von Count / Duck (voice)
- Richard Hunt
- Gladys the Cow / Forgetful Jones / Don Music (voice)
- Kevin Clash
- Elmo / Hoots the Owl (voice)
- David Rudman
- (voice) (archive footage)
- Pam Arciero
- Grundgetta
- Cheryl Blaylock
- Blue Honker
- Edward G. Christie
- Ernie (Assistant)
- Michael Earl
- (voice) (as Mike Davis)
- James J. Kroupa
- (voice) (credit only)
- Noel MacNeal
- (voice)
- Brian Meehl
- Purple Honker
- Kathryn Mullen
- Green Honker
- Karen Prell
- (voice) (archive footage)
- Caroly Wilcox
- (voice) (archive footage)
- Bryant Young
- (voice)
- Jim Henson
- Ernie / Singer (segment "One Banana") / Kermit the Frog (voice) (archive footage)
- Emilio Delgado
- Luis
- Sonia Manzano
- Maria
- Carl Banks
- Self
- John Candy
- Yosh Shmenge
- Celia Cruz
- Self
- Jane Curtin
- Self
- Danny DeVito
- Self
- Keith Hernandez
- Self
- Mark Ingram Sr.
- Self
- Jeremy Irons
- Self
- Gordon Jackson
- Self
- Madeline Kahn
- Self
- Ladysmith Black Mambazo
- Themselves
- Sean Landeta
- Self
- Wynton Marsalis
- Self
- Jean Marsh
- Self
- Andrea Martin
- Edith Prickley
- Karl Nelson
- Self
- Itzhak Perlman
- Self
- Rhea Perlman
- Self
- Paul Reubens
- Pee-Wee Herman
- Pete Seeger
- Self
- Paul Simon
- Self
- James Taylor
- Self
- Mookie Wilson
- Self
- Joe Williams
- Self
- Jeanie Bennett
- Polly Darton
- Jim Kweskin
- Vocals
- Jim Thurman
- Singer 'Calcutta Joe'
- Steve Zuckerman
- Singer 'Ten Turtles'
- Fran Brill
- Polly Darton (segment "Wavin' Goodbye to You with My Heart")
- Martin P. Robinson
- Dinger
