- Joseph Gordon-Levitt
- Self - Host
- Pam Arciero
- Goldilocks / Grundgetta / Telly Monster (hand) / Additional Muppets (voice)
- Jennifer Barnhart
- Gladys the Cow / Mama Bear / Zoe (voice)
- Tau Bennett
- Additional Muppets
- Warrick Brownlow-Pike
- Gonger / Panicked Purple Monster / Banner Holding Pigeon / Mumford's Bunny / Gold Sandwich Eating Monster / Green Anything Muppet at Hooper's (voice)
- Tyler Bunch
- Dish / Louie / Letter A (voice)
- Leslie Carrara-Rudolph
- Abby Cadabby
- Frankie Cordero
- Rudy
- Stephanie D'Abruzzo
- Betty Lou / Pink Jacket / Little Bird / Prairie Dawn (voice)
- Ryan Dillon
- Don Music / Elmo / Lefty the Salesman / Roosevelt Franklin (hand) (voice)
- Stacey Gordon
- Julia
- Chris Thomas Hayes
- Hoots the Owl
- Eric Jacobson
- Bert / Grover / Guy Smiley / Harvey Kneeslapper / Oscar the Grouch / Two-Headed Monster (Left Head) (voice)
- John Kennedy
- The Amazing Mumford
- Peter Linz
- Captain Vegetable / Ernie / Herry Monster (voice)
- Spencer Lott
- Additional Muppets
- Noel MacNeal
- Additional Muppets
- Paul McGinnis
- Additional Muppets
- Carmen Osbahr
- Ovejita / Rosita / Spoon (voice)
- Martin P. Robinson
- Mr. Snuffleupagus / Old MacDonald / Telly Monster (voice)
- David Rudman
- Baby Bear / Cookie Monster / Letter Y / Sully / Two-Headed Monster (Right Head) (voice)
- Matt Vogel
- Biff / Big Bird / The Count / Forgetful Jones / Kermit the Frog / Mr. Johnson / Sherlock Hemlock (voice)
- Fran Brill
- Self
- Sterling K. Brown
- Self
- Elvis Costello
- Self
- Whoopi Goldberg
- Self
- Norah Jones
- Self
- Solange
- Self
- Patti LaBelle
- Self
- Itzhak Perlman
- Self
- Nile Rodgers
- Self
- Caroll Spinney
- Self / Big Bird
- Debra Spinney
- Self
- Meghan Trainor
- Self
- Alison Bartlett
- Gina
- Linda Bove
- Linda
- Emilio Delgado
- Luis
- Olamide Faison
- Miles
- Bill Irwin
- Mr. Noodle
- Chris Knowings
- Chris / Roosevelt Franklin (voice)
- Loretta Long
- Susan
- Suki Lopez
- Nina
- Sonia Manzano
- Maria
- Bob McGrath
- Bob
- Alan Muraoka
- Alan
- Roscoe Orman
- Gordon
- Nitya Vidyasagar
- Leela
- Greer Barnes
- Special Extra
- Lorenzo Giliberti
- Special Extra
- Clementine Kline
- Special Extra
- Hazel Anne Raymundo
- Special Extra
- Jennifer Auberjonois
- Additional Muppets
- Elizabeth Duff
- Additional Muppets
- Chris Guttadaro
- The Baker
- Jim Henson
- The Baker
- Samantha McDanel
- Additional Muppets
- Paul McGinnis
- Additional Muppets
- Mike Ancas
- Additional Muppets
- Josh Gad
- Self
- Zoe Glick
- Hopscotch Girl
- Joey Mazzarino
- Murray Monster
- Kate McKinnon
- Self
- Bryant Young
- Mr. Snuffleupagus (back half)
