- Janel Parrish
- Janel Parrish the Host
- Frank Welker
- Self - Voice of Fred Jones and Scooby Doo
- Grey Griffin
- Self - Voice of Daphne
- Matthew Lillard
- Self - Voice of Shaggy
- Kate Micucci
- Self - Voice of Velma
- Karamo Brown
- Costume Designer Carter
- Brendan Jennings
- Arlo Davidsonman, Team Scooby
- Vadym Krasnenko
- Snow Ghost
- Olivia Liang
- Shaggy's Martial Arts Trainer
- Mary Lynn Rajskub
- Producer Brynn
- Baron Vaughn
- Bram Penobscot, Scooby's Vocal Coach
- Lindsey Alena
- Additional Voices
- Diedrich Bader
- Batman
- Fred Tatasciore
- Snow Ghost
- Dominic Catrambone
- Additional Voices
- Cheri Oteri
- Lazlo the Mechanic
- Jerry Beck
- Self - Animation Historian and Author
- Seth Green
- Self - Actor, Scooby-Doo 2: Monsters Unleashed
- Tony Cervone
- Self - Director, Scoob & Showrunner, Mystery Incorporated
- David Silverman
- Self - Director and Producer, The Simpsons
- Tom Sito
- Self - Animator, Historian
- Mitch Watson
- Self - Showrunner, Mystery Incorporated
- 'Weird Al' Yankovic
- Self - Singer, Songwriter, Accordionist
- Joseph Barbera
- Self - Hanna-Barbara Co-Founder
- Silvia Lake
- WB Security Guard #1
- Iwao Takamoto
- Self - Hanna-Barbara Animator
