- Fred Armisen
- Stuart / Various (archive footage)
- Edward Asner
- Mr. Wilson
- Dan Aykroyd
- Rick Phillips
- Vanessa Bayer
- Rosa / Elise (archive footage)
- John Belushi
- Kevin
- Aidy Bryant
- Various
- Beth Cahill
- Denise Swerski
- Kieran Culkin
- Student
- Macaulay Culkin
- Tommy Arnold
- Jane Curtin
- Mrs. Loud
- Rachel Dratch
- Debbie Downer
- Abby Elliott
- Nancy
- Jimmy Fallon
- Jarret
- Chris Farley
- Mrs. O'Connor / Todd O'Connor (archive footage)
- Will Ferrell
- Cornelius Blake / Various (archive footage)
- Carrie Fisher
- Joan Loud
- Will Forte
- Terry
- Ana Gasteyer
- Mrs. Blake / Martha Stewart / Various (archive footage)
- Joseph Gordon-Levitt
- Stephen
- Shelly Gossman
- Volunteer (segment "Free Thanksgiving Dinner")
- Christopher Guest
- Ed's Father
- Bill Hader
- Pastor Mike / Devin / Various (archive footage)
- Phil Hartman
- Frankenstein (segment "Thanksgiving Greetings")
- Anne Hathaway
- Mary (segment "Free Thanksgiving Dinner")
- Josh Hutcherson
- Trey (segment "Thanksgiving Guest")
- Chris Kattan
- Nathan (segment "Bird Family")
- Tim Kazurinsky
- Mr. Landlord
- Taran Killam
- Tyler / Various (archive footage)
- Jon Lovitz
- Tonto (segment "Thanksgiving Greetings")
- Julianna Margulies
- Karen (segment "Bird Family")
- Kate McKinnon
- Various
- Seth Meyers
- Jonathan Feinstein / Various (archive footage)
- Lorne Michaels
- Self (segment "Paul Simon Monologue")
- Kyle Mooney
- Various
- Garrett Morris
- Police Officer
- Bobby Moynihan
- Todd / Various (archive footage)
- Brittany Murphy
- Holly
- Eddie Murphy
- Mister Robinson
- Bill Murray
- Mr. Loud
- Mike Myers
- Pat Arnold
- Kevin Nealon
- Weekend Update Anchor / Tarzan (archive footage)
- Michael Patrick O'Brien
- Volunteer (segment "Free Thanksgiving Dinner")
- Gwyneth Paltrow
- Tammy (segment "Family Dinner")
- Chris Parnell
- Paul (segment "Bird Family")
- Nasim Pedrad
- Various
- Amy Poehler
- Michelle / Elizabeth (archive footage)
- Gilda Radner
- Jean Loud
- Jeremy Renner
- Craig (segment "The Californians")
- Tim Robinson
- Glenn / Various (archive footage)
- Chris Rock
- Rapper (segment "Nikey Turkey")
- Adam Sandler
- Various
- Horatio Sanz
- Uncle Frank / Gobi (archive footage)
- Martin Short
- Ed Grimley
- Paul Simon
- Self (segment "Paul Simon Monologue")
- Robert Smigel
- Carl Wollarski
- Pamela Stephenson
- Mrs. Wilson
- Cecily Strong
- Various
- Jason Sudeikis
- Phil
- Kenan Thompson
- Trey / Various (archive footage)
- George Wendt
- Bob Swerski
- Kristen Wiig
- Penelope / Judith (archive footage)
- Luke Wilson
- Mark (segment "Debbie Downer")
- Jack Handey
- Narrator (segment "Fuzzy Memories")
