- Andy Samberg
- Self - Host
- Fred Armisen
- Nathanial Snerpus
- Dan Aykroyd
- Self
- Jim Belushi
- Virgil's Boss
- John Belushi
- Self
- Josh Brolin
- Kyle
- Steve Carell
- Japanese Michael Scott
- Dana Carvey
- Garth
- Tom Davis
- Self
- James Downey
- Ben Samberg / Newspaper Vendor (archive footage) (uncredited)
- Christine Ebersole
- Hidden Photo Waitress
- Will Ferrell
- The German Intellectual
- Will Forte
- MacGruber
- Ricky Gervais
- Self
- Christopher Guest
- Director
- Bill Hader
- Various
- Rich Hall
- Virgil
- Darrell Hammond
- Richard Dreyfuss / Regis Philbin (archive footage) (uncredited)
- Phil Hartman
- Doorman
- Jonah Hill
- Self
- Jan Hooks
- Lady
- Becca Lish
- Maraka
- Norm MacDonald
- Burt Reynolds
- Madonna
- Self
- Peyton Manning
- Self
- Eddie Murphy
- Self
- Bill Murray
- Neil Wasserheim
- Mike Myers
- Wayne
- Laraine Newman
- Self
- Chris Parnell
- Self
- Bill Paxton
- Self
- Joe Piscopo
- Allen Funt
- Amy Poehler
- Various
- Gilda Radner
- La Dolce Gilda
- Jerry Seinfeld
- Self
- Harry Shearer
- Gerald
- Martin Short
- Lawrence
- Kevin Spacey
- Christopher Walken / Walter Matthau (archive footage) (uncredited)
- Jason Sudeikis
- Japanese Jim Halpert
- Kenan Thompson
- Japanese Stanley Hudson
- Justin Timberlake
- Self
- Andy Warhol
- Self
- Kristen Wiig
- Various
- Casey Wilson
- Office Co-Worker
