- Jim J. Bullock
- Adam Seymour 'Queer Duck' Duckstein
- Jackie Hoffman
- Lola Buzzard
- Kevin Michael Richardson
- Stephen Arlo 'Openly' Gator / Additional Voices (voice)
- Billy West
- Bi-Polar Bear / Additional Voices (voice)
- Estelle Harris
- Mrs. Duckstein
- Mark Hamill
- Vendor
- Tim Curry
- Peccary
- Andy Dick
- Elizabeth Taylor / Rex ('Regina') (voice)
- Chris Cox
- Michael Jackson
- Audrey Wasilewski
- Rosie O'Donnell
- Maurice LaMarche
- Oscar Wildcat / Martin Duckstein / Additional Voices (voice)
- Jeff Bennett
- Rev. Vandergelding / Additional Voices (voice)
- Kevin Chamberlin
- Additional Voices
- Barbara Goodson
- Elizabeth Taylor
- Howard Hoffman
- Additional Voices
- Nick Jameson
- Additional Voices
- Tress MacNeille
- Dr. Laura Schlessinger / Barbra Streisand / Additional Voices (voice)
- Debi Mae West
- Joan Rivers / Additional Voices (voice)
- April Winchell
- Additional Voices
- Bruce Vilanch
- Bruce Vilanch
- Conan O'Brien
- Conan O'Brien
- David Duchovny
- Tiny Jesus
