- Curtis Arnott
- (voice)
- Ken Ashcorp
- (voice) (as Kenny Ashcorp)
- Bryon Beaubien
- Wario
- Duke Beersponge
- (voice)
- Danny Boyer
- (voice)
- Phil Brute
- (voice)
- Kira Buckland
- Paula
- Brittney Butler
- (voice)
- Cailen Denton
- Ness
- Lucien Dodge
- (voice)
- Scott Frerichs
- (voice)
- Kelli Giggles
- (voice)
- Corey Holland
- (voice)
- Richard Jao
- (voice)
- Anthony Kroze Kresky
- Mr. Saturn
- Crystal Lonnquist
- (voice)
- Francine L. Farley
- (voice) (as Francine Louise)
- Deven Christian Mack
- (voice) (as a different name)
- Faye Mata
- (voice)
- Dorn Matthews
- (voice)
- Marin M. Miller
- Samus
- Jesse Nowack
- (voice) (as a different name)
- Johnny Rivera
- (voice)
- Michael Ruocco
- Poo
- Anthony Sardinha
- (voice)
- Noah Scammon
- (voice)
- Lawrence Simpson
- (voice)
- Johnny Staggs
- (voice)
- Carolyn Stevens
- (voice)
- Corinne Sudberg
- Pichu
- Marc Swint
- (voice)
- Nick Tiner
- Fox McCloud
- Edwyn Tiong
- Jeff
- Joshua Tomar
- (voice)
- Kimlinh Tran
- (voice)
- Chris Zito
- Bowser / Falco Lombardi (voice)
- Martin Billany
- Waluigi / Dr. Stewart (voice) (as Martin 'LittleKuriboh' Billany)
- Max Gilardi
- Ness' Dad
- Joe Gran
- (voice) (as Joe 'Ukinojoe' Gran)
- Harry Partridge
- (voice) (as Harry 'HappyHarry' Partridge)
- Weston Durant
- (voice) (as Weston 'Kajetokun' Durant)
- Joey Blanchette
- Peppy Hare / Diddy Kong (voice) (uncredited)
- Chris Niosi
- Dog
