- Rene Aranda
- Thorn
- James Burzelic
- Narrator
- T.C. De Witt
- Professor Jenkins / Abraham Van Jenkins / Mr. Jenkins / Xeno-Jenkins / The Gentleman Ghost (voice)
- Travis Joe Dixon
- Shaggy / Little Shaggy / Norvus / Shaggy X / Rogers (voice)
- Chase Hofer
- Red Flammé / Dr. Red Flam / Mascot Bear (voice)
- Joy Regullano
- Velma / Nightmare Velma / Little Velma / Velma X / Velaria (voice)
- Brent Rose
- Fred / Little Fred / Captain Caveman / Fred X / Freddy / Frederick (voice)
- Dani J. Scott
- Daphne / Little Daphne / Daphneo / Blake / Daphne Connor (voice)
- Scott Woodard
- Scooby
