- Ashleigh Ball
- Applejack / Rainbow Dash / Nurse Redheart (voice)
- Andrea Libman
- Pinkie Pie / Fluttershy (voice)
- Rebecca Shoichet
- Sunset Shimmer / Twilight Sparkle - singing (voice)
- Tabitha St. Germain
- Rarity / Princess Luna / Vice Principal Luna (voice)
- Tara Strong
- Twilight Sparkle / Princess Twilight Sparkle (voice)
- Nicole Oliver
- Princess Celestia
- Cathy Weseluck
- Spike
- Kathleen Barr
- Trixie Lulamoon
- Shannon Chan-Kent
- Wallflower Blush / Pinkie Pie - singing (voice) (as Shannon Chan Kent)
- James Kirk
- Micro Chips
- Ingrid Nilson
- Maud Pie
- Kazumi Evans
- Rarity
