- Uma Thurman
- The Bride / Beatrix Kiddo (archive footage)
- Lucy Liu
- O-Ren Ishii (Cottonmouth)
- Vivica A. Fox
- Vernita Green (Copperhead)
- Michael Madsen
- Budd (Sidewinder)
- Daryl Hannah
- Elle Driver (California Mountain Snake)
- David Carradine
- Bill (Snake Charmer)
- Julie Dreyfus
- Sofie Fatale
- Chiaki Kuriyama
- Gogo Yubari
- Shin'ichi Chiba
- Hattori Hanzo
- Chia-Hui Liu
- Johnny Mo / Pai Mei (as Gordon Liu)
- Michael Parks
- Earl McGraw / Esteban Vihaio
- Michael Bowen
- Buck
- Jun Kunimura
- Boss Tanaka
- Kenji Ôba
- Bald Guy (Sushi Shop)
- Yuki Kazamatsuri
- Proprietor
- James Parks
- Edgar McGraw
- Sakichi Sato
- Charlie Brown
- Jonathan Loughran
- Trucker
- Yoshiyuki Morishita
- Tokyo Businessman
- Tetsurô Shimaguchi
- Crazy 88 #1 (Miki)
- Kazuki Kitamura
- Crazy 88 #2
- Yôji Tanaka
- Crazy 88 #3
- Issei Takahashi
- Crazy 88 #4
- So Yamanaka
- Crazy 88 #5
- Juri Manase
- Crazy 88 #6 (Girl)
- Akaji Maro
- Boss Ozawah
- Gorô Daimon
- Boss Honda
- Shun Sugata
- Boss Benta
- Jinzhan Zhang
- Boss Orgami
- Xiaohui Hu
- Young 88 (Spanked Boy)
- Ambrosia Kelley
- Nikki Bell
- Sachiko Fujii
- The 5, 6, 7, 8's
- Yoshiko Yamaguchi
- The 5, 6, 7, 8's
- Ronnie Yoshiko Fujiyama
- The 5, 6, 7, 8's
- Shu Lan Tuan
- Okinawa Airline Ticket Agent
- Ai Maeda
- O-Ren (anime sequence)
- Naomi Kusumi
- Boss Matsumoto (anime sequence)
- Hikaru Midorikawa
- Pretty Riki (anime sequence)
- Bo Svenson
- Reverend Harmony
- Jeannie Epper
- Mrs. Harmony
- Stephanie L. Moore
- Joleen
- Shana Stein
- Erica
- Caitlin Keats
- Janeen
- Christopher Allen Nelson
- Tommy Plympton
- Reda Beebe
- Lucky
- Sid Haig
- Jay
- Larry Bishop
- Larry Gomez
- Laura Cayouette
- Rocket
- Clark Middleton
- Ernie
- Claire Smithies
- Clarita
- Helen Kim
- Karen Kim
- Perla Haney-Jardine
- B.B.
- Victoria Lucai
- Trixie
- Venessia Valentino
- 1st Grade Teacher
- Thea Rose
- Melanie Harrhouse
- William Paul Clark
- Soda Jerk
- Stevo Polyi
- Tim
- Al Manuel Douglas
- Marty Kitrosser
- Patricia Silva
- Hooker #1
- Maria Del Rosario Gutiérrez
- Hooker #2
- Sonia Angelica Padilla Curiel
- Hooker #3
- Veronica Janet Martinez
- Hooker #4
- Lucia Cruz Marroquin
- Hooker #5
- Citlati Guadalupe Bojorquez
- Hooker #6
- Graciela Salazar Mendoza
- Hooker #7
- Maria de Lourdes Lombera
- Hooker #8
- Jorge Silva
- Bartender / Pimp
- Aaron Rafael
- Boss Matsumoto
