- Jesse James Jr.
- Jesse James
- Diana Reed
- Lucille James, Jesse's Daughter (epilogue)
- Jack Neil
- Robert Standing - Lucille's Sweetheart (epilogue)
- Marguerite Hungerford
- Zee Mimms - Mrs. Jesse James
- Ralph Johnson
- Judge Bowman
- Hortense Espey
- Mrs. Bowman
- William Baker
- Jesse's Step-Father
- Mrs. Cart
- Jesse's Mother
- Frances Coffey
- Susan - Jesse's Sister
- Elmo Red Fox
- Chief Red Fox
- Gilbert Holmes
- Pee Wee Holmes - Rodeo Bulldogger
