- Robert Clarence Irwin
- Â Self 17 episodes, 2021
- Chandler Powell
- Â Self 11 episodes, 2021
- Bindi Irwin
- Â Self 11 episodes, 2021
- Terri Irwin
- Â Self 11 episodes, 2021
- Grace Warrior Irwin Powell
- Â Self 4 episodes, 2021
- Luke Reavley
- Â Self / ... 3 episodes, 2021
- Toby Millyard
- Â Self - Head of Crocs 3 episodes, 2021
- Rosemary Booth
- Â Self - Chief of Staff- Australia Wildlife Hospital 2 episodes, 2021
- Ludo Valenza
- Â Self - Veterinarian 2 episodes, 2021
- Stella Irwin
- Â Self 2 episodes, 2021
- Piggy Irwin
- Â Self 2 episodes, 2021
- Renee Australia Zoo
- Â Self - Rescue Unit 1 episode, 2021
- Ashleigh Clare
- Â Self - Australia Zoo Human Resources and Recruitment Coordinator 1 episode, 2021
- Penny Lenhan-Hawser
- Â Self - Head Keeper and Ranger 1 episode, 2021
- Lou Rendell
- Â Self - Australia Zoo 1 episode, 2021
- Sui Irwin
- Â Self 1 episode, 2021
- Riley Mannion
- Â Self 2 episodes, 2021
- Steve Irwin
- Â Self (archive footage) 5 episodes, 2021
