- The Marx Brothers
- (as The Four Marx Brothers)
- Groucho Marx
- Professor Quincy Adams Wagstaff
- Harpo Marx
- Pinky
- Chico Marx
- Baravelli
- Zeppo Marx
- Frank Wagstaff
- Thelma Todd
- Connie Bailey
- David Landau
- Jennings
- Bobby Barber
- Speakeasy Patron
- Reginald Barlow
- Retiring College President
- Vince Barnett
- Speakeasy Patron
- Sheila Bromley
- Wagstaff's Receptionist
- E.H. Calvert
- Professor in Wagstaff's Study
- Edgar Dearing
- Speakeasy Bartender
- Robert Greig
- Biology Professor Giving Lecture
- Theresa Harris
- Laura - Connie's Maid
- Edward LeSaint
- Professor in Wagstaff's Study
- Florine McKinney
- Peggy Carrington
- Nat Pendleton
- MacHardie - Darwin Player
- Lee Phelps
- Referee
- James Pierce
- Ed Mullen - Darwin Player
- Frank Rice
- Doorman at Speakeasy
- Syd Saylor
- Speakeasy Patron at Slot Machine
- Arthur Sheekman
- Typing Sportswriter
- Ben Taggart
- Police Officer
- Phil Tead
- Football Broadcaster
