- William Patterson
- Strong Bad / The Cheat / Announcer / Eh Steve / Homsar (voice) (as DaWillstanator)
- Joey Turner
- Homestar Runner / Coach Z / Bubs / Wheelchair (voice)
- Logan Ridenbaugh
- Strong Mad / King of Town (voice)
- Kira Buckland
- Twilight Sparkle / Rainbow Dash (voice)
- Lizzie Freeman
- Applejack / Rarity (voice)
- Tanya Furness
- Marzipan
- Tyler Hildebrand
- Strong Sad
- Sheila M. Lin
- Princess Celestia / Giggling Ponies (voice)
- Logan Miller
- Bulk Biceps
- Wish Lotus
- Apple Bloom
- Pinkie Rose
- Sweetie Belle
- Shari Weygandt
- Fluttershy
- Cherry SeaVA
- Scootaloo
