- Sean Connery
- James Bond
- Ursula Andress
- Honey Ryder
- Joseph Wiseman
- Dr. No
- Jack Lord
- Felix Leiter
- Bernard Lee
- M.
- Anthony Dawson
- Professor Dent
- Zena Marshall
- Miss Taro
- John Kitzmiller
- Quarrel
- Eunice Gayson
- Sylvia
- Lois Maxwell
- Miss Moneypenny
- Peter Burton
- Major Boothroyd
- Yvonne Shima
- Sister Lily
- Michel Mok
- Sister Rose
- Marguerite LeWars
- Annabel Chung - Photographer
- William Foster-Davis
- Superintendent
- Dolores Keator
- Mary
- Reggie Carter
- Jones
- Louis Blaazer
- Pleydell-Smith
- Colonel Burton
- General Potter
- Abbot Anderson
- Crab Key Guard
- Jack Arrow
- Le Cercle Patron
- Nigel Bernard
- Le Cercle Patron
- Keith Binns
- Crab Key Guard
- Chris Blackwell
- Henchman Jumping Off Dock into Water
- Kes Chin
- Dragon Guard
- Anthony Chinn
- Decontamination Technician
- Diana Coupland
- Honey Ryder - Singing Voice
- Eric Coverley
- 1st Three Blind Mice Assassin
- Reggie de Beer
- Casino Patron
- Alicia Deane
- Woman in Dr. No's Lair
- Charles Edghill
- 2nd Three Blind Mice Assassin
- Margaret Ellery
- Stewardess
- Joe Enrikie
- Le Cercle Patron
- Lancelot Evans
- Crab Key Dock Guard
- Peter Evans
- Le Cercle Patron
- Hilary Farish
- Stewardess
- Charles Gilliard
- Le Cercle Waiter
- Alan Gold
- Croupier at La Cercle
- Arthur Goodman
- Le Cercle Patron
- Victor Harrington
- Le Cercle Patron
- John Hatton
- Radio Operator
- George Hilsdon
- Le Cercle Patron
- Billy John
- Le Cercle Patron
- Gerry Judge
- Le Cercle Waiter
- Pat Judge
- Le Cercle Waiter
- Juba Kennerley
- Le Cercle Patron
- Bettine Le Beau
- Professor Dent's Secretary
- Arnold Lee
- Le Cercle Patron
- Byron Lee
- Singer at Puss Feller's
- George Leech
- Decontamination Technician
- Dan Lester
- Le Cercle Patron
- Rick Lester
- Guard
- Henry Lopez
- 3rd Three Blind Mice Assassin
- Keith Lyn
- Vocalist - the Dragonaires
- Louis Marriott
- Dragon Guard
- Colin McKenzie
- Le Cercle Patron
- Count Prince Miller
- Nightclub Dancer
- Stanley Morgan
- Concierge at Le Cercle
- Tim Moxon
- Professor John Strangways
- Malou Pantera
- Hotel Receptionist
- Willie Payne
- Johnny - Hotel Worker
- Lester Pendergast
- Puss Feller
- Carol Reckford
- Dent's Boat Captain
- Milton Reid
- Dr. No's Guard
- Robert Rietty
- John Strangways / Superintendent Duff (voice) (uncredited)
- Adrian Robinson
- Hearse Driver
- Carey Robinson
- Guard with Loudspeaker
- Harold Sanderson
- Le Cercle Patron
- Bunny Seaman
- Le Cercle Patron
- John Sefton
- Le Cercle Croupier
- Maxwell Shaw
- Communications Operator
- Bob Simmons
- James Bond in Gunbarrel Sequence
- Frank Singuineau
- Hotel Waiter
- John Smart
- Le Cercle Patron
- Graham Tonbridge
- Le Cercle Patron
- Nikki Van der Zyl
- Honey Ryder / Sylvia Trench / Various (voice) (uncredited)
- Robert Vossler
- Le Cercle Waiter
- Pearl Walters
- Le Cercle Patron
- John Wilder
- Le Cercle Cashier
