- Claudette Colbert
- Cleopatra
- Warren William
- Julius Caesar
- Henry Wilcoxon
- Marc Antony
- Joseph Schildkraut
- Herod
- Ian Keith
- Octavian
- Gertrude Michael
- Calpurnia
- C. Aubrey Smith
- Enobarbus
- Irving Pichel
- Apollodorus
- Arthur Hohl
- Brutus
- Edwin Maxwell
- Casca
- Ian Maclaren
- Cassius
- Eleanor Phelps
- Charmion
- Leonard Mudie
- Pothinos
- Grace Durkin
- Iras
- Ferdinand Gottschalk
- Glabrio
- Claudia Dell
- Octavia
- Harry Beresford
- Soothsayer
- Jayne Regan
- Lady Vesta
- William Farnum
- Lepidus
- Lionel Belmore
- Fidius
- Florence Roberts
- Lady Flora
- Richard Alexander
- Gen. Philodemas
- Celia Ryland
- Lady Leda
- William V. Mong
- Court Physician
- Robert Warwick
- Gen. Achillas
- George Walsh
- Courier
- Kenneth Gibson
- Scribe
- Wedgwood Nowell
- Scribe
- Bruce Warren
- Scribe
- Robert Seiter
- Aelius
- Edgar Dearing
- Convict
- Agnes de Mille
- Dancer
- Victor Alexander
- Cleopatra's Retinue
- Jimmy Aye
- Slave
- Zita Baca
- Handmaiden
- Joe Bacon
- Fan Bearer
- Malcolm Ball
- Minor Role
- V. Baptiste
- Cleopatra's Retinue
- Lucille Battle
- Dancer / Slave Girl (uncredited)
- Leon Beaumon
- Egyptian Guard
- Martin Beaumon
- Egyptian
- Carmelita Black
- Dancer / Slave Girl (uncredited)
- Carlyle Blackwell Jr.
- Undetermined Role
- N. Blake
- Cleopatra's Retinue
- Marjorie Bonner
- Roman Girl
- Marion Booker
- Dancer / Slave Girl (uncredited)
- Mildred Boyd
- Dancer / Slave Girl (uncredited)
- Everett Brown
- Cleopatra's Bodyguard
- Lenora Brown
- Dancer / Slave Girl (uncredited)
- George Bruggeman
- Slave
- Daisy Bufford
- Dancer / Slave Girl (uncredited)
- Edmund Burns
- Roman
- Horace B. Carpenter
- Roman
- John Carradine
- Roman Citizen / Party Guest / Soldier (voice) (uncredited)
- Olga Celeste
- Slave Girl
- Jack Clisby
- Cleopatra's Retinue / Swimmer (uncredited)
- Thomas Cobb
- Cleopatra's Retinue
- Bobbe Cronin
- Girl
- Mae Digges
- Dancer / Slave Girl (uncredited)
- Ecki
- A Leopard
- Elias English
- Fan Bearer
- Mary Fahrney
- Undetermined Role
- Tom Farrell
- Cleopatra's Retinue
- Myrtle Fortune
- Dancer / Slave Girl (uncredited)
- Jerry Frank
- Slave
- Elmer Frye
- Cleopatra's Retinue
- Bill Gibson
- Cleopatra's Retinue
- Ruth Givens
- Dancer / Slave Girl (uncredited)
- Catherine Grayson
- Dancer / Slave Girl (uncredited)
- Lawrence Green
- Cleopatra's Retinue
- Bob Hall
- Roman Soldier
- Lucille Hall
- Dancer / Slave Girl (uncredited)
- Edgar Hampton
- Canopy Bearer
- Neal Hart
- Slave
- Bob Hayden
- Cleopatra's Retinue
- Cleo Herndon
- Dancer / Slave Girl (uncredited)
- Shep Houghton
- Roman Soldier
- Patsy Hunter
- Dancer / Slave Girl (uncredited)
- Julanne Johnston
- Undetermined Role
- Darby Jones
- Fan Bearer
- Edmund Jones
- Nubian Slave
- Jilda Keeling
- Undetermined Role
- Lucyle Keeling
- Girl
- Al Knight
- Cleopatra's Retinue
- Nicholai Konovaloff
- Roman General
- Lillian Leighton
- Onlooker at Procession
- Rosalie Lincoln
- Dancer / Slave Girl (uncredited)
- Timothy J. Lonergan
- Roman General
- Wilfred Lucas
- Roman Greeting Antony
- Sherley Mack
- Cleopatra's Retinue
- Mary MacLaren
- Roman Woman
- Henry Martin
- Cleopatra's Retinue
- Ray Martin
- Bodyguard to Cleopatra
- Alice Meadows
- Dancer / Slave Girl (uncredited)
- Ellen Meadows
- Dancer / Slave Girl (uncredited)
- John Merton
- Roman Guard
- Juanita Moore
- Dancer / Slave Girl (uncredited)
- Perry Moore
- Cleopatra's Retinue
- Charles Morris
- Cicero
- G.B. Morrow
- Bodyguard to Cleopatra
- Jack Mulhall
- Roman Greeting Antony
- David Niven
- Slave
- Jack O'Connor
- Cleopatra's Retinue
- Ed Patrick
- Canopy Bearer
- Ernestine Porter
- Dancer / Slave Girl (uncredited)
- A.J. Prather
- Cleopatra's Retinue
- T.L. Pratt
- Cleopatra's Retinue
- Hal Price
- Onlooker at Procession
- Rae Randall
- (uncredited)
- Harry Raven
- Slave
- Hazel Reese
- Dancer / Slave Girl (uncredited)
- Gus Robinson
- Cleopatra's Retinue
- Louise Robinson
- Dancer / Slave Girl (uncredited)
- Mrs. Tom Rooney
- Undetermined Role
- John Roy
- Roman Soldier
- Jack Rutherford
- Drussus - Model Builder
- Carl Saxe
- Roman Soldier
- Rube Schaffer
- Undetermined Role
- Ynez Seabury
- Undetermined Role
- William Slater
- Cleopatra's Retinue
- Phillips Smalley
- Roman
- Ernie Smith
- Roman Soldier
- T.G. Smith
- Nubian Guard
- Terrence Spears
- Bodyguard to Cleopatra
- Henry Stayden
- Canopy Bearer
- Lawrence Stewart
- Cleopatra's Retinue / Swimmer (uncredited)
- Jas Stowers
- Bodyguard to Cleopatra
- Winona Taylor
- Dancer / Slave Girl (uncredited)
- Earl Thompkins
- Cleopatra's Retinue
- Marjorie Timm
- Girl
- Alma Travers
- Dancer / Slave Girl (uncredited)
- Earl Turman
- Nubian Guard
- Earl Turnham
- Cleopatra's Retinue
- Dale Van Sickel
- Roman Soldier
- Paul Venerable
- Canopy Bearer
- Colleen Ward
- Dancer / Slave Girl (uncredited)
- Bryant Washburn Jr.
- Undetermined Role
- Bryant Washburn
- Undetermined Role
- Blue Washington
- Nubian Guard
- Charles Washington
- Cleopatra's Retinue
- Henry Washington
- Fan Bearer
- Genevieve Webb
- Dancer / Slave Girl (uncredited)
- John Webb
- Cleopatra's Retinue
- Dorothy White
- Dancer
- Dot Williams
- Dancer / Slave Girl (uncredited)
