- Brick Bronsky
- Roger Smith
- Lisa Gaye
- Professor Holt
- Leesa Rowland
- Victoria
- Michael Kurtz
- Yoke
- Scott Resnick
- Dean Okra
- Jacki Moen
- Diane / Bald Subhumanoid (as Jacquelyn Rene Moen)
- Robert Dawson
- Murray
- Phil Rivo
- Harvey / Malathion Man
- Erica Frank
- Tour Guide
- Sharon E. Gardner
- Mother with Baby
- Bea Lindgren
- Grandmother
- Lorraine Parchment
- Yoke's Girlfriend
- Troy Fromin
- Yoke's Sidekick
- Tyler Bowe
- Yoke's Sidekick
- Paul Borghese
- Baseketball Coach / Toxic Avenger Assistant Director (as Julian Paul Borghese)
- Amy Hilbrich
- Basketball Player
- Brad Roth
- Chain Smoking Student / Squirrels - The Gang
- Nello Scadato
- Nurse Chave
- Jean Stewart
- Nurse Pony
- Gerrard Course
- Doctor
- Lindsey Dawkins
- Plaine White Rapper
- Darla Haun
- Plain White Rapper
- Lily Hayes Kaufman
- Pre-Pubescent Professor Holt / Child at Birthday Party
- Jason Vance Taylor
- Green Man
- Alex Pirnie
- Mutant Squirrel
- William J. Kulzer
- Chief Brundt
- Kaptain
- Decomposed Green Guy
- Madison Monk
- Toxic Avenger Director
- Greta Rubens
- French Teacher
- Jon Albert
- Fungus Mungus
- Patricia Kaufman
- Nurse at Birthday Party
- Jerri Greene
- Screaming Tromatized Housewife
- Patti Williford
- Screaming Tromatized Housewife
- Thomas Perry
- The Toxic Avenger
- Wendy Burnell
- Squirrels - The Gang
- Shane Christopher
- Squirrels - The Gang
- Ana Debasa
- Squirrels - The Gang / Bald Subhumanoid
- Jesse Cole Johnson
- Squirrels - The Gang
- Andre Lambert
- Squirrels - The Gang
- Jeanne LeGrand
- Squirrels - The Gang
- Julie Nine
- Squirrels - The Gang
- Louis Ortiz
- Squirrels - The Gang
- Glorivic Parong
- Squirrels - The Gang
- Suzanne Solari
- Squirrels - The Gang
- Lisa Star
- Squirrels - The Gang
- Saint Teresa Stone
- Squirrels - The Gang
- Billie Taylor
- Squirrels - The Gang
- Veronica Stork
- Squirrels - The Gang
- Elizabeth Young
- Squirrels - The Gang
- Kristina Krofft
- Virgin Subhumanoid
- Maraya Chase
- Subhumanoid Squirrel Feeder
- Sophia Orkulas
- Subhumanoid Squirrel Feeder
- Traci Mann
- Ms. Rowland's Stand-In
- Walt Kubiak
- Tromaville Nerd
- Martin Sokol
- Tromaville Nerd
- Mark Richardson
- Motorcycle Couple / Rock Band - 674IF
- Diana C. Skinner
- Motorcycle Couple
- Sheri Belfert
- Bald Subhumanoid
- Bonnie Clarke
- Bald Subhumanoid
- Heather Forsyth
- Bald Subhumanoid
- Susan Jones
- Bald Subhumanoid
- Trinity Loren
- Bald Subhumanoid
- Sharon Mitchell
- Bald Subhumanoid
- Phoebe Phaigh
- Bald Subhumanoid
- Sandy Ruthowski
- Bald Subhumanoid
- Nicole Vasilopoulos
- Bald Subhumanoid
- Ryan Jones
- Rock Band - 674IF
- Andy Jones
- Rock Band - 674IF
- John Bausch
- Rock Band - 674IF
- Gilbran Chong
- Child at Birthday Party
- Charlotte Kaufman
- Child at Birthday Party
- Lisbeth Kaufman
- Child at Birthday Party
- Shanah Richardson
- Child at Birthday Party
- Sherie Richardson
- Child at Birthday Party
- Cosimo Sherman
- Child at Birthday Party
- Josh Greenie
- Young Boy on Rollerskates
- Brent Lichman
- Young Boy on Rollerskates
- Wes Lichtman
- Young Boy on Rollerskates
- Rocky Sherman
- Young Boy on Rollerskates
- Jessi Woodhull
- Young Boy on Rollerskates
- Shannon Doyle McCool
- Squirrel, Nerd
- Shelby Shepard
- Professor Janice Jones
- Bullet Valmont
- Squirrel, Humanoid
- Liz Ard
- Squirrels - The Gang / Humanoid (uncredited)
- Michael Cadava
- Squirrels - The Gang / Subhumanoid (uncredited)
- Corey Hawk
- Young Boy on Rollerskates
- Michael Herz
- Helicopter Pilot
- Lloyd Kaufman
- Helicopter Pilot
- Cynthia Windham
- Lady who sees 'it's a bird'
