- John Lund
- Alfonso D'Este
- Paulette Goddard
- Lucretia Borgia
- Macdonald Carey
- Cesare Borgia
- Raymond Burr
- Michelotto
- Donald Randolph
- Tiziano
- Albert Dekker
- Vanetti
- Charles Dayton
- Bastino
- Anthony Caruso
- Captain of the Guard
- Dick Foote
- Negligent Sentry
- John Sutton
- Prince Bisceglie
- William Farnum
- Conti Peruzzi
- Kate Drain Lawson
- Gemma
- Nicholas Joy
- Chamberlain
- Fritz Leiber
- Filippo
- Rose Hobart
- Lady Eleanora
- Douglas Spencer
- False Physician
- Nestor Paiva
- Mayor
- Frank Puglia
- Bolfi
- Houseley Stevenson
- Councillor
- Robert Greig
- Councillor
- Don Beddoe
- Councillor
- Kirk Alyn
- Guard
- James Anderson
- Guard
- Gordon Arnold
- Courtier
- John Arnold
- (uncredited)
- Tony Barr
- (uncredited)
- Edward Biby
- Citizen
- Robert Bice
- Guard
- John Bleifer
- Doctor
- Nan Boardman
- (uncredited)
- James Carlisle
- Citizen
- Gordon B. Clarke
- Prisoner
- Henry Corden
- Scout
- Victor Desny
- Doctor
- Jimmie Dundee
- Assassin
- Morgan Farley
- Treasurer
- Franklyn Farnum
- Bit Role
- Charles Fogel
- Citizen
- Billy Gilbert
- Beppo
- Chuck Hamilton
- Troop Captain
- James Harrison
- (uncredited)
- Len Hendry
- Guard
- George Holmes
- Troop Captain
- John David Horsley
- Troop Captain
- Robert Kellard
- Guard
- Peter Mamakos
- Ferrara's Crossbowman
- Myron Marks
- (uncredited)
- Irene Martin
- (uncredited)
- Ed Millard
- Herald
- Clayton Moore
- Long Bowman
- Hugh Murray
- Surgeon
- Gordon Nelson
- Doctor
- Milicent Patrick
- (uncredited)
- Gil Perkins
- Huntsman
- Gilman Rankin
- Scout
- Sally Rawlinson
- Lady in Waiting
- George Robotham
- Assassin
- Victor Romito
- Captain of Borgia Guards
- Charles Sherlock
- (uncredited)
- Erica Strong
- Lady in Waiting
- John Vosper
- (uncredited)
- Richard Webb
- Prisoner
- Dean White
- Sentry
- Ian Wolfe
- Councillor
- George Zoritch
- Gianni
