- Mathew Baynton
- William 'Bill' Shakespeare / Lord Burghley / English Messenger / Customs Official
- Simon Farnaby
- Earl of Croydon / Juan Domingo / Sausage / Dmitri Alexandrovitch / Fur Seller
- Martha Howe-Douglas
- Anne Hathaway / Molly / Spanish Courtier / Body Collector
- Jim Howick
- Christopher Marlowe / Gabriel Montoya / Cynical Jester / Palace Doorman / Mysterious Man / Even Grubbier Thief / Party Planner
- Laurence Rickard
- Sir Francis Walsingham / Lope Lopez / Stand-Up Jester / Chatty Guard / Slightly Late Courtier / Ian / Hanging Criminal / Chicken Drumstick
- Ben Willbond
- King Phillip II of Spain / Earl of Southampton / Grubby Thief / Alexander Dimitrievitch / Head of Guards
- David Crow
- Ramon: Cockney Player / Sergeant
- Jamie Demetriou
- Sergio: Cockney Player
- Richard Atwill
- Seve: Cockney Player
- John Henry Falle
- Miguel: Cockney Player
- Andrew Young
- Spanish Guard 1
- Elliot Young
- Spanish Guard 2
- Damian Lewis
- Sir Richard Hawkins
- Michael Collin
- Despatch Rider
- Susy Kane
- Lady-in-waiting
- Clare Thomson
- Lady-in-waiting
- Susie Donkin
- Lady-in-waiting
- Helen McCrory
- Queen Elizabeth I
- Lucy Brown
- Susanne
- Sophie Sanderson
- Judith
- Reggie Moore
- Hamnet
- Tom Cox
- Mortal Coil
- Joey Ellis
- Mortal Coil
- Will Rastall
- Mortal Coil
- Warnock Kerr
- Earl of Worcester
- Gareth Tunley
- Clipboard Guard / Priest
- Justin Edwards
- Sir Francis Drake
- Rufus Jones
- Sir Walter Raleigh
- George Sawyer
- Thirsty Courtier / Pie Stall Owner
- Matthew Kitchen
- Fire Eater
- Bridget Vercambre
- Prostitute
- Tom Meeten
- Spanish Chamberlain
- Stephen Greif
- Spanish Adviser
- Robin Hodges
- London Thief / Bull Inn Landlord
- Richard Glover
- Catholic Contact
- Andy Gillies
- Boris
- Jared Morgan
- Protestant Bishop
- Richard Czornowol
- Hawker / Assistant Body Collector
- Darren Edge
- Monk 2
- Umer Farooq
- Spanish Goon
- Mark Hindman Smith
- Bridge-Leaping Peasant / Londoner (as Mark H. Smith)
- Tim Willbond
- Tudor Tim
- William Bale
- Walsingham's Men
- Richard Banks
- Catholic Monk
- Nigel Black
- Monk 1
- David Blockley
- Walsingham's Men
- Calum C. Sutton
- Walsingham's Man
- Paul Cavendish
- Courtier
- Grant Crookes
- Courtier
- Michael Degnan
- Londoner
- Richie Donaldson
- Thug / plague Victim (uncredited)
- Patrick Durham
- Courtier
- Christine Estima
- Spanish Lady 9
- Sam Exley
- Spanish Courtier
- Rod Glenn
- Builder
- Cris Haris
- Palace Guest
- Kate Hawley
- Lady Wessex
- George Hewer
- Spanish Servant #1
- Amanda Higson
- Courtier
- Samantha Hindman
- Quill & Rapier Landlady / Londoner (uncredited)
- Kevin Hudson
- Tavern Keeper
- David Macey
- Customs Officer
- Monty Mclaren-Clark
- Street Urchin
- Alexander Mounsey
- Palace Guard
- Shaun Newnham
- Man in Tavern
- Taavi Peelo
- Londoner
- Jack Philips
- Statue / Spanish Guard (uncredited)
- Ian Ray-White
- Plague Victim
- Nig Richards
- Courtier
- Hugh Robb
- Street Urchin
- Miley Roberts
- Courtier
- Julio Romeo
- Courtier
- Olesja Sidorovich
- Lady-in-waiting 6
- Neil Tattersall
- Courtier
- Orson Thomas
- Walsingham's Men
- Lyndsey Walker
- Portrait Artist
- Paula Ann Williams
- Londerer
- Brian Woodward
- Courtier
