- David Shea
- Batman / Bruce Wayne (voice)
- J Tyler Pennington
- Killer Croc
- Beau Roberts
- Commissioner Jim Gordon
- Brittney Cassidy
- Barbara Gordon / Oracle / Young Bruce Wayne / Martha Wayne (voice)
- Hunter Cassidy
- The Joker
- Jake Donald Truitt
- Scarecrow / Alfred Pennyworth (voice)
- Ashlee Lawhorn
- Poison Ivy
- Curtis Combrink
- The Penguin
- Beth Metcalf
- Harley Quinn
- David Perry
- The Riddler
- Raevin Koster
- Vicki Vale
- Carl Baumgartner
- Poison Ivy Thugs
- Sergei Scozio
- Penguin Thugs
- Simon Freitas
- The Riddler Thugs
- Kristopher N Heaton
- Joker Thugs
- Tylor Chapman
- GCPD Officer #1
- Brandon Sekula
- GCPD Officer #2
- Cyndee Hunter
- GCPD Officer #3
- Bryan J Mangam
- GCPD Officer #4
- Liz Staples
- GCPD Dispatcher
- David J. Paul
- Thomas Wayne
- Nate Taylor
- Arkham Asylum Guard #1
- Jake Reynolds
- Arkham Asylum Guard #2
- Andrew Underwood
- Funeral Celebrant
