- Brice Armstrong
- Tokugawa Ieyasu
- Ed Blaylock
- Yagyu Munenori
- John Burgmeier
- Chikuma Koshiro
- Chris Cason
- Kazamachi Shogen
- Colleen Clinkenbeard
- Hotarubi
- Justin Cook
- Yashamaru
- Greg Dulcie
- Hattori Hanzo
- R. Bruce Elliott
- Azuki Rousai
- Juli Erickson
- Ogen Iga
- Kyle Hebert
- Hanzo the First
- Jeremy Inman
- Udono Josuke
- Grant James
- Danjo Kouga
- Bill Jenkins
- Narrator
- Jamie Marchi
- Youth Ogen
- Andy Mullins
- Nankoubou Tenkai
- Daniel Penz
- Kasumi Renbu
- Jennifer Seman
- Akeginu
- J. Paul Slavens
- Amayo Jingoro
- Mark Stoddard
- Yakushiji Tenzen
- Matthew Tompkins
- Youth Danjo
- Eric Vale
- Jimushi Jyubei
- Dave Willis
- Carriage Bear
