- Isa Baschwitz
- Self
- Mary Bos
- Self - Anne's Friend
- Kenneth Branagh
- Narration
- Janny Brandes-Brilslijper
- Self - Dutch Resistance Prisoner
- Glenn Close
- Diary Readings
- Rose De Liema
- Self - Westerbork Prisoner Aug / Sept 1944 (as Rose de Liema)
- Sal De Liema
- Self - Westerbork Prisoner Aug / Sept 1944 (as Sal de Liema)
- Buddy Elias
- Self - Anne's cousin / Self- Otto Frank's nephew (as Bernd Elias)
- Bloeme Evers
- Self - Margot's classmate / Self - Westerbork Prisoner Aug / Sept 1944 (as Bloeme Evers-Emden)
- Alice Frank
- Self - Otto's Mother
- Anne Frank
- Self
- Helene Frank
- Self - Otto's Sister
- Margot Frank
- Self
- Otto Frank
- Self
- Robert Frank
- Self - Otto 's Brother
- Miep Gies
- Self
- Kitty Gokkel-Egyedi
- Self - Anne's Friend
- Hanneli Goslar
- Self
- Abraham Holländer
- Self - Edith's Father
- Charlotte Kaletta
- Self - Fritz's fiancée
- Juultje Ketellapper
- Self - Anne's Friend
- Nelson Mandela
- Self - South African President 1994 - 1999, Political Prisoner 1964 - 1990
- Frieda Menco
- Self - Westerbork Survivor / Self - Westerbork Prisoner July / Sept 1944
- Laureen Nussbaum
- Self
- Peter Pepper
- Self - Fritz Pfeffer's son
- Werner Peter Pfeffer
- Self - Fritz Pfeffer's son
- Hannah Pick-Goslar
- Self
- Joely Richardson
- Diary Readings
- Rosa Stern
- Self - Edith's Mother
- Rie Swillens
- Self - Anne's Friend
- Rachel Van Amerongen-Frankfoorder
- Self - Westerbork Prisoner June / Sept 1944 (as Rachel van Amerongen-Frankfoorder)
- Henk van Beersekamp
- Self
- Martha van den Berg
- Self - Anne's Friend
- Lucie van Dijk
- Self - Anne's Friend
- Jacqueline van Maarsen
- Self - Anne's friend
- Elisabeth van Wijk-Voskuijl
- Self - Employee Hitler
- Hans Wijnberg
- Self - Tenant
- Edith Frank
- Self
- Jan Gies
- Self
- Joseph Goebbels
- Self
- Hermann Göring
- Self
- Rudolf Hess
- Self
- Johannes Kleiman
- Self
- Victor Kugler
- Self
- Fritz Pfeffer
- Self
- Auguste van Pels
- Self
- Herman van Pels
- Self
- Peter van Pels
- Self
