- Richard Arlen
- Cheshire Cat
- Roscoe Ates
- Fish
- William Austin
- Gryphon
- Gary Cooper
- White Knight
- Leon Errol
- Uncle Gilbert
- Louise Fazenda
- White Queen
- W.C. Fields
- Humpty-Dumpty
- Alec B. Francis
- King of Hearts
- Richard 'Skeets' Gallagher
- Rabbit
- Cary Grant
- Mock Turtle
- Lillian Harmer
- Cook
- Raymond Hatton
- Mouse
- Charlotte Henry
- Alice
- Sterling Holloway
- Frog
- Edward Everett Horton
- Mad Hatter
- Roscoe Karns
- Tweedledee
- Baby LeRoy
- Joker
- Mae Marsh
- Sheep
- Polly Moran
- Dodo Bird
- Jack Oakie
- Tweedledum
- Edna May Oliver
- Red Queen
- May Robson
- Queen of Hearts
- Charles Ruggles
- March Hare
- Jackie Searl
- Dormouse
- Alison Skipworth
- Duchess
- Ned Sparks
- Caterpillar
- Ford Sterling
- White King
- Billy Barty
- White Pawn / The Baby (uncredited)
- Billy Bevan
- Two of Spades
- Colin Campbell
- Garden Frog
- Jack Duffy
- Leg of Mutton
- Harry Ekezian
- First Executioner
- Meyer Grace
- Third Executioner
- Ethel Griffies
- Miss Simpson the Governess
- Colin Kenny
- The Clock
- Charles McNaughton
- Five of Spades
- Patsy O'Byrne
- The Aunt
- George Ovey
- Plum Pudding
- Will Stanton
- Seven of Spades
- Joe Torillo
- Second Executioner
