- Johnny Yong Bosch
- Additional Voices
- Katsumi Chô
- Parmenion
- Mari Devon
- Additional Voices
- Dorothy Elias-Fahn
- Renee
- Toshio Furukawa
- Alexander
- Yoko Honna
- Renee
- Shûichi Ikeda
- Philip
- Satoshi Katôgi
- Hephaestion (young)
- Kiyoshi Kobayashi
- Aristotle
- Yuri Lowenthal
- Ptolemy
- Dave Mallow
- Additional Voices
- Michael McConnohie
- Aristotle
- Phil Meyers
- Additional Voices
- Kôki Miyata
- Alexander (young)
- Masaru Motegi
- Officer A
- Ryûsei Nakao
- Hephaestion
- Liam O'Brien
- Alexander
- Joe Ochman
- Additional Voices
- Jamieson Price
- King Philip II
- Sam Riegel
- Philip
- Takahiro Sakurai
- Philip (young)
- Stephanie Sheh
- Additional Voices
- Daisaku Shinohara
- King Philip II
- Amy Smith
- Additional Voices
- Keiichi Sonobe
- Doctor
- Steve Staley
- Hephaestion
- Kan Tanaka
- Coachman
- Kirk Thornton
- Parmenion
- Takayuki Yamaguchi
- Ptolemy (young)
- Kazuhiro Yamaji
- Ptolemy
- Kazuko Yanaga
- Olympias
- Clara York
- Additional Voices
