- William L. Thorne
- Tom Lincoln
- Lucille La Verne
- Mid-Wife
- Helen Freeman
- Nancy Hanks Lincoln
- Otto Hoffman
- Offut
- Walter Huston
- Abraham Lincoln
- Edgar Dearing
- Armstrong
- Una Merkel
- Ann Rutledge
- Russell Simpson
- Lincoln's Employer
- Charles Crockett
- Sheriff
- Kay Hammond
- Mary Todd Lincoln
- Helen Ware
- Mrs. Edwards
- E. Alyn Warren
- Stephen A. Douglas / General Grant
- Jason Robards Sr.
- Herndon
- Gordon Thorpe
- Tad Lincoln
- Ian Keith
- John Wilkes Booth
- Cameron Prud'Homme
- John Hay - Secretary to the President
- James Bradbury Sr.
- Gen. Winfield Scott
- James Eagles
- Young Soldier
- Oscar Apfel
- Secretary of War Stanton
- Frank Campeau
- General Sheridan
- Hobart Bosworth
- General Robert E. Lee
- Henry B. Walthall
- Col. Marshall
- Hank Bell
- Townsman in Offut's Store
- Maurice Black
- Conspirator
- Ed Brady
- Confederate Courier
- Robert Brower
- (uncredited)
- Kernan Cripps
- Conspirator
- Count Cutelli
- Baby Abraham Lincoln Crying Sounds
- Emilia Da Prato
- Vocalist
- Bess Flowers
- Undetermined Role
- Mary Forbes
- Actress
- Francis Ford
- Sheridan's Aide
- Beatrice Hagen
- Vocalist
- Robert Homans
- A Senator - One of Lincoln's Advisors
- Jane Keckley
- Matchmaker
- Robert Keith
- Union Courier
- Henry Kolker
- New Englander
- Ralph Lewis
- Member of Lincoln's Cabinet
- George MacQuarrie
- Member of Lincoln's Cabinet
- Scott Seaton
- Minor Role
- Carl Stockdale
- Member of Lincoln's Cabinet
- Harry Stubbs
- Undetermined Role
- Kathrin Clare Ward
- Townswoman at Ann's Death
